Astrin Antibody - BSA Free
Novus Biologicals | Catalog # NB100-74638
![Immunocytochemistry/ Immunofluorescence: Astrin Antibody [NB100-74638]](https://resources.rndsystems.com/images/products/Astrin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74638-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Astrin Antibody [NB100-74638]")
Key Product Details
Validated by
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Format
Product Specifications
Immunogen
Clonality
Host
Isotype
Scientific Data Images for Astrin Antibody - BSA Free
![Immunoprecipitation: Astrin Antibody [NB100-74638]](https://resources.rndsystems.com/images/products/Astrin-Antibody-Immunoprecipitation-NB100-74638-img0002.jpg "Immunoprecipitation: Astrin Antibody [NB100-74638]")
Immunoprecipitation: Astrin Antibody [NB100-74638]
Immunoprecipitation: Astrin Antibody [NB100-74638] - Detection of Human Astrin/MAP126 by Western Blot of Immunoprecipitants. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Astrin/MAP126 antibody NB100-74638 used for IP at 3 mcg/mg lysate. Astrin/MAP126 was also immunoprecipitated by another rabbit anti-Astrin/MAP126 antibody and NB100-74639, which recognize downstream epitopes. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: Astrin Antibody [NB100-74638] -
Immunohistochemistry: Astrin Antibody [NB100-74638] - SKA3 localisation is not abrogated due to the lack of Astrin.(A) Representative deconvolved images show SKA3 enrichment at kinetochores of HeLa cells treated with Control or Astrin siRNA. Cells were arrested in metaphase using MG132 for 1 hr prior to fixation & were immunostained with antibodies against Astrin & SKA3 & CREST antiserum (to mark centromeres) & stained with DAPI for DNA. Cropped images show Astrin or SKA3 enrichment at kinetochores. (B) Representative images show SKA3 enrichment at kinetochores of HeLa cells treated with Astrin siRNA before transfection with eukaryotic protein expression vectors encoding YFP-Astrin (WT, 4A or ∆70, as indicated). Cells were arrested in metaphase using MG132 for 1 hr prior to fixation & were immunostained with antibodies against GFP (for detecting YFP-Astrin) & SKA3 & CREST antiserum & co-stained with DAPI for DNA. Cropped images show YFP-Astrin or SKA3 enrichment at kinetochores. Scale as indicated. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31808746), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Immunocytochemistry/ Immunofluorescence: Astrin Antibody - BSA Free [NB100-74638] -
SKA3 localisation is not abrogated due to the lack of Astrin.(A) Representative deconvolved images show SKA3 enrichment at kinetochores of HeLa cells treated with Control or Astrin siRNA. Cells were arrested in metaphase using MG132 for 1 hr prior to fixation and were immunostained with antibodies against Astrin and SKA3 and CREST antiserum (to mark centromeres) and stained with DAPI for DNA. Cropped images show Astrin or SKA3 enrichment at kinetochores. (B) Representative images show SKA3 enrichment at kinetochores of HeLa cells treated with Astrin siRNA before transfection with eukaryotic protein expression vectors encoding YFP-Astrin (WT, 4A or ∆70, as indicated). Cells were arrested in metaphase using MG132 for 1 hr prior to fixation and were immunostained with antibodies against GFP (for detecting YFP-Astrin) and SKA3 and CREST antiserum and co-stained with DAPI for DNA. Cropped images show YFP-Astrin or SKA3 enrichment at kinetochores. Scale as indicated. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31808746), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Astrin Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence
Immunoprecipitation
Formulation, Preparation, and Storage
Purification
Formulation
Format
Preservative
Concentration
Shipping
Stability & Storage
Background: Astrin
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional Astrin Products
Product Documents for Astrin Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Astrin Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Astrin Antibody - BSA Free
Powered by Bioz
Customer Reviews for Astrin Antibody - BSA Free
There are currently no reviews for this product. Be the first to review Astrin Antibody - BSA Free and earn rewards!
Have you used Astrin Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Astrin Antibody - BSA Free
-
Q: We recently bought Astrin antibody from your company (catalog # NB100-74638, date of purchase 24/01/2012). We did not realize this antibody is not for Western blotting until we received it, and we failed to optimize the antibody detection. Because the other Astrin antibodies are still available from Novus Biologicals, we are thinking to purchase another Astrin antibody. However, in order to ensure the successful use of antibody for our research, we would like to ask which Astrin antibody is guaranteed for Western blotting and possibly for immunofluorescence using human cells ? Also, in the case if the antibody does not work, would you consider the refunding or sending us the alternative antibody?
A: We have 6 antibodies that match your requirements, and they can be viewed on our website by searching for Astrin and narrowing your results using the filters on the left-hand side of the screen. We offer a 100% guarantee for the species and applications listed on the products' datasheets and are happy to provide refunds or replacements in the event that they do not perform as promised!