Ataxin-3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32083

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (93%), Sheep (92%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Ataxin-3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Ataxin-3. The exact sequence is proprietary.

Reactivity Notes

Mouse (88%), Rat (82%), Pig (89%).

Localization

Nucleus matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ataxin-3 Antibody - BSA Free

Western Blot: Ataxin-3 Antibody [NBP1-32083]

Western Blot: Ataxin-3 Antibody [NBP1-32083]

Western Blot: Ataxin-3 Antibody [NBP1-32083] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Ataxin-3 Antibody [NBP1-32083]

Immunocytochemistry/ Immunofluorescence: Ataxin-3 Antibody [NBP1-32083]

Immunocytochemistry/Immunofluorescence: Ataxin-3 Antibody [NBP1-32083] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ataxin 3 protein stained by Ataxin 3 antibody diluted at 1:250.Blue: Hoechst 33342 staining.
Western Blot: Ataxin-3 Antibody [NBP1-32083]

Western Blot: Ataxin-3 Antibody [NBP1-32083]

Western Blot: Ataxin-3 Antibody [NBP1-32083] - Various whole cell extracts (30 ug) were separated by 10 % SDS-PAGE, and blotted with Ataxin 3 antibody diluted by 1:1000
Western Blot: Ataxin-3 Antibody [NBP1-32083]

Western Blot: Ataxin-3 Antibody [NBP1-32083]

Western Blot: Ataxin-3 Antibody [NBP1-32083] - Ataxin 3 antibody detects Ataxin 3 protein by western blot analysis. 293T mock transfected (-) or transfected with a construct expressing human Ataxin 3. 10 % SDS-PAGE Ataxin 3 antibody dilution: 1:20000
Immunocytochemistry/ Immunofluorescence: Ataxin-3 Antibody [NBP1-32083]

Immunocytochemistry/ Immunofluorescence: Ataxin-3 Antibody [NBP1-32083]

Immunocytochemistry/Immunofluorescence: Ataxin-3 Antibody [NBP1-32083] - 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ataxin 3 protein stained by Ataxin 3 antibody diluted at 1:250. Blue: Hoechst 33342 staining.

Applications for Ataxin-3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

Assay dependent

Western Blot

1:500-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Ataxin-3

Machado-Joseph disease, also known as spinocerebellar ataxia-3, is an autosomal dominant neurologic disorder. The protein encoded by this gene contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is one cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. (provided by RefSeq)

Alternate Names

AT3, Ataxin3, ATXN3, JOS, MJD1, SCA3

Gene Symbol

ATXN3

Additional Ataxin-3 Products

Product Documents for Ataxin-3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Ataxin-3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Deubiquitinating Enzymes (DUBs)

Citations for Ataxin-3 Antibody - BSA Free

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Protocols

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