ATF1 Antibody (23GB4325)

Novus Biologicals | Catalog # NBP3-50335

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB4325
View all ATF1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human ATF1 (Uniprot: P18846)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATF1 Antibody (23GB4325)

ATF1 Antibody (23GB4325)

Immunocytochemistry/ Immunofluorescence: ATF1 Antibody (23GB4325)[NBP3-50335] -

Immunocytochemical staining of HepG2 cells with ATF1 antibody (1:1,000). Nuclei were stained blue with DAPI; ATF1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
ATF1 Antibody (23GB4325)

Flow Cytometry: ATF1 Antibody (23GB4325)[NBP3-50335] -

Flow cytometric analysis of ATF1expression in HepG2 cells using ATF1 antibody (1:2,000). Green, isotype control; red, Activating transcription factor 1.
ATF1 Antibody (23GB4325)

Western Blot: ATF1 Antibody (23GB4325)[NBP3-50335] -

Western blotting analysis using ATF1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for ATF1 Antibody (23GB4325)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATF1

ATF2 (Activating Transcription Factor 2, CREBP, HB16, CREB2, TREB7) is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.

Long Name

Activating Transcription Factor 1

Alternate Names

TREB36

Gene Symbol

ATF1

Additional ATF1 Products

Product Documents for ATF1 Antibody (23GB4325)

Certificate of Analysis

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Product Specific Notices for ATF1 Antibody (23GB4325)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

TGF-beta Signaling Pathways
TGF-beta Signaling Pathway Thumbnail