ATG3/APG3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-37894

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ATG3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: KGKALEVAEYLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWQWATGEELKVKAYLPTGKQFLVTKNVPCYKRCKQMEYSDELEAIIEEDDGDGGWVDTYHNTGITGITEAVKEITLENKDNIRLQDCSALCE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATG3/APG3 Antibody - BSA Free

Immunohistochemistry-Paraffin: ATG3/APG3 Antibody [NBP2-37894]

Immunohistochemistry-Paraffin: ATG3/APG3 Antibody [NBP2-37894]

Immunohistochemistry-Paraffin: ATG3/APG3 Antibody [NBP2-37894] - Staining of human testis shows high expression.
Immunohistochemistry: ATG3/APG3 Antibody [NBP2-37894]

Immunohistochemistry: ATG3/APG3 Antibody [NBP2-37894]

Immunohistochemistry: ATG3/APG3 Antibody [NBP2-37894] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: ATG3/APG3 Antibody [NBP2-37894]

Immunohistochemistry-Paraffin: ATG3/APG3 Antibody [NBP2-37894]

Immunohistochemistry-Paraffin: ATG3/APG3 Antibody [NBP2-37894] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: ATG3/APG3 Antibody [NBP2-37894]

Immunohistochemistry-Paraffin: ATG3/APG3 Antibody [NBP2-37894]

Immunohistochemistry-Paraffin: ATG3/APG3 Antibody [NBP2-37894] - Staining in human testis and skeletal muscle tissues using anti-ATG3 antibody. Corresponding ATG3 RNA-seq data are presented for the same tissues.

Applications for ATG3/APG3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATG3

FUNCTION: GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy. SUBUNIT: Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. SUBCELLULAR LOCATION: Cytoplasm. ALTERNATIVE PRODUCTS: 2 named isoformsproduced by alternative splicing. TISSUE SPECIFICITY: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. SIMILARITY: Belongs to the ATG3 family.

Long Name

Autophagy Related 3 Homolog

Alternate Names

APG3L, Apg3p, PC3-96

Gene Symbol

ATG3

UniProt

Q9NT62 (Human)

Additional ATG3 Products

Product Documents for ATG3/APG3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ATG3/APG3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy

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Protocols

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FAQs

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