ATG4C Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89043

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ATG4C Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EEARHPDLQGITIYVAQDCTVYNSDVIDKQSASMTSDNADDKAVIILVPVRLGGERTNTDYLEFVKGILSLEYCVGIIGGKPKQSYYFAGFQDDSLIYMDPHYCQSFV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATG4C Antibody - BSA Free

Western Blot: ATG4C Antibody [NBP1-89043]

Western Blot: ATG4C Antibody [NBP1-89043]

Western Blot: ATG4C Antibody [NBP1-89043] - Analysis in control (vector only transfected HEK293T lysate) and ATG4C over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: ATG4C Antibody [NBP1-89043]

Immunocytochemistry/ Immunofluorescence: ATG4C Antibody [NBP1-89043]

Immunocytochemistry/Immunofluorescence: ATG4C Antibody [NBP1-89043] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: ATG4C Antibody [NBP1-89043]

Immunohistochemistry-Paraffin: ATG4C Antibody [NBP1-89043]

Immunohistochemistry-Paraffin: ATG4C Antibody [NBP1-89043] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Applications for ATG4C Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATG4C

ATG4C is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.

Alternate Names

APG4 autophagy 4 homolog C, APG4 autophagy 4 homolog C (S. cerevisiae), APG4C, ATG4 autophagy related 4 homolog C (S. cerevisiae), AUT (S. cerevisiae)-like 1, cysteine endopeptidase; AUT-like 1, cysteineendopeptidase (S. cerevisiae), AUTL1, AUTL3APG4-C, AUT-like 1, cysteine endopeptidase, AUT-like 3 cysteine endopeptidase, autophagin-3, Autophagy-related cysteine endopeptidase 3, Autophagy-related protein 4 homolog C, cysteine protease ATG4C, EC 3.4.22, EC 3.4.22.-, FLJ14867

Gene Symbol

ATG4C

Additional ATG4C Products

Product Documents for ATG4C Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ATG4C Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ATG4C Antibody - BSA Free

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Protocols

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