ATG9B Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-92806

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all ATG9B Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 715-924 of human ATG9B (NP_001303985.1). HPLWRPPGHSSKFLGHLWGRVQQDAAAWGATSARGPSTPGVLSNCTSPLPEAFLANLFVHPLLPPRDLSPTAPCPAAATASLLASISRIAQDPSSVSPGGTGGQKLAQLPELASAEMSLHVIYLHQLHQQQQQQEPWGEAAASILSRPCSSPSQPPSPDEEKPSWSSDGSSPASSPRQQWGTQKARNLFPGGFQVTTDTQKEPDRASCTD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATG9B Antibody - Azide and BSA Free

Western Blot: ATG9B AntibodyAzide and BSA Free [NBP2-92806]

Western Blot: ATG9B AntibodyAzide and BSA Free [NBP2-92806]

Western Blot: ATG9B Antibody [NBP2-92806] - Western blot analysis of extracts of HUH-7 cells, using ATG9B antibody (NBP2-92806) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western Blot: ATG9B AntibodyAzide and BSA Free [NBP2-92806]

Western Blot: ATG9B AntibodyAzide and BSA Free [NBP2-92806]

Western Blot: ATG9B Antibody [NBP2-92806] - Western blot analysis of extracts of various cell lines, using ATG9B antibody (NBP2-92806) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
ATG9B Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: ATG9B Antibody - Azide and BSA Free [NBP2-92806] -

Immunocytochemistry/ Immunofluorescence: ATG9B Antibody - Azide and BSA Free [NBP2-92806] - Immunofluorescence analysis of human placenta cells using ATG9B Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Applications for ATG9B Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATG9B

FUNCTION: May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. SUBUNIT: Interacts with GABRG2, TUBA1, ULK1 and NSF. Interacts with beta-tubulin and GPHN. SUBCELLULAR LOCATION: Intracytoplasmic membrane. Cytoskeleton. Largely associated with intracellular membrane structures including the Golgi apparatus and post-synaptic cisternae. Colocalizes with microtubules. SIMILARITY: Belongs to the MAP1 LC3 family.

Alternate Names

APG9L2APG9-like 2, ATG9 autophagy related 9 homolog B (S. cerevisiae), autophagy-related protein 9B, endothelial nitric oxide synthase antisense, nitric oxide synthase 3 antisense, Nitric oxide synthase 3-overlapping antisense gene protein, NOS3ASautophagy 9-like 2 protein, Protein sONE, SONEFLJ14885

Gene Symbol

ATG9B

Additional ATG9B Products

Product Documents for ATG9B Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ATG9B Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for ATG9B Antibody - Azide and BSA Free

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  • Q: How much is the molecular weight of ATG9B protein?

    A: The predicted molecular weight of ATG9B protein is 101kDa

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