ATP6V1E1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-14335

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ATP6V1E1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: KEKQIEQQKKIQMSNLMNQARLKVLRARDDLITDLLNEAKQRLSKVVKDTTRYQVLLDGLVLQGLYQLLEPRMIVRCRKQDFPLVKAAVQKAIPMYKIATKNDVDVQIDQESYLPEDIAGGVEIYNGDRKIKVSNTLESRLDLIAQQMMPEVRGALFGANANRKF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATP6V1E1 Antibody - BSA Free

Western Blot: ATP6V1E1 Antibody [NBP2-14335]

Western Blot: ATP6V1E1 Antibody [NBP2-14335]

Western Blot: ATP6V1E1 Antibody [NBP2-14335] - Analysis in human cell line SK-MEL-30.
Immunocytochemistry/ Immunofluorescence: ATP6V1E1 Antibody [NBP2-14335]

Immunocytochemistry/ Immunofluorescence: ATP6V1E1 Antibody [NBP2-14335]

Immunocytochemistry/Immunofluorescence: ATP6V1E1 Antibody [NBP2-14335] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: ATP6V1E1 Antibody [NBP2-14335]

Immunohistochemistry-Paraffin: ATP6V1E1 Antibody [NBP2-14335]

Immunohistochemistry-Paraffin: ATP6V1E1 Antibody [NBP2-14335] - Analysis in human cerebral cortex and skeletal muscle tissues using NBP2-14335 antibody. Corresponding ATP6V1E1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ATP6V1E1 Antibody [NBP2-14335]

Immunohistochemistry-Paraffin: ATP6V1E1 Antibody [NBP2-14335]

Immunohistochemistry-Paraffin: ATP6V1E1 Antibody [NBP2-14335] - Staining of human cerebral cortex shows moderate granular cytoplasmic positivity in neuropil.
Immunohistochemistry-Paraffin: ATP6V1E1 Antibody [NBP2-14335]

Immunohistochemistry-Paraffin: ATP6V1E1 Antibody [NBP2-14335]

Immunohistochemistry-Paraffin: ATP6V1E1 Antibody [NBP2-14335] - Staining of human kidney shows strong membranous positivity in cells in tubules.
Immunohistochemistry-Paraffin: ATP6V1E1 Antibody [NBP2-14335]

Immunohistochemistry-Paraffin: ATP6V1E1 Antibody [NBP2-14335]

Immunohistochemistry-Paraffin: ATP6V1E1 Antibody [NBP2-14335] - Staining of human pancreas shows moderate cytoplasmic positivity in islets of Langerhans.
Immunohistochemistry-Paraffin: ATP6V1E1 Antibody [NBP2-14335]

Immunohistochemistry-Paraffin: ATP6V1E1 Antibody [NBP2-14335]

Immunohistochemistry-Paraffin: ATP6V1E1 Antibody [NBP2-14335] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Applications for ATP6V1E1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATP6V1E1

ATP6V1E1 encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome. [provided by RefSeq]

Alternate Names

ATP6E2ATP6V1E, ATP6E31kDa subunit, ATPase, H+ transporting, lysosomal (vacuolar proton pump) 31kD, ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E isoform 1, ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1, H+-transporting ATP synthase chain E, vacuolar, p31, Vacuolar proton pump subunit E 1, V-ATPase 31 kDa subunit, V-ATPase subunit E 1, V-ATPase, subunit E, Vma4, V-type proton ATPase subunit E 1

Gene Symbol

ATP6V1E1

Additional ATP6V1E1 Products

Product Documents for ATP6V1E1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ATP6V1E1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for ATP6V1E1 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review ATP6V1E1 Antibody - BSA Free and earn rewards!

Have you used ATP6V1E1 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies