ATPase Antibody

Novus Biologicals | Catalog # NBP1-52178

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Key Product Details

Species Reactivity

Human, Canine, Rabbit

Applications

Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ATPase Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EGTVKLKTIDN corresponding to internal region according to NP_001362.2.

Epitope

C-EGTVKLKTIDN

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Applications for ATPase Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000
Application Notes
WB: Not yet tested - Our standard WB protocol would not expect to reliably detect proteins as large as the calculated size of 515 kDa band according to NP_001362.2.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATPase

Na,K-ATPase is an integral membrane protein complex that hydrolyzes ATP to maintain the transmembrane gradients of Na+ and K+ found in most mammalian cells. The enzyme is comprised of an alpha and beta subunit. The alpha-polypeptide has been shown to be the catalytically active subunit, whereas the beta-polypeptide appears to be necessary for the assembly and transport of the sodium pump to the plasma membrane. Na,K-ATPase a1 subunit, in brain, is expressed in both neuronal and non-neuronal cells.

Alternate Names

ATPase, axonemal, dynein, axonemal, heavy chain 8, dynein, axonemal, heavy polypeptide 8, FLJ25850, FLJ36115, FLJ36334, HDHC9

Entrez Gene IDs

1769 (Human)

Gene Symbol

DNAH8

UniProt

NP_001362.2 (Human)

Additional ATPase Products

Product Documents for ATPase Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ATPase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

ATPases

Citations for ATPase Antibody

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FAQs for ATPase Antibody

Showing  1 - 4 of 4 FAQs Showing All

  • Q: I have phosphate in my enzyme. What can I do?

    A: You can dialyse or desalt the enzyme into a phosphate-free buffer. Alternatively, you can use a special resin (PiBind) to remove the phosphate.

  • Q: I have 5% DMSO in my assay. Can I use PiColorLock Gold?

    A: Yes, the reagent is designed for drug screening work and other situations that require DMSO.

  • Q: I have a high background in my ATPase assay and I definitely do not have free phosphate in my sample

    A: This is almost always due to inadequate mixing of the special stabilizer with the sample and detection reagent. Make sure the stabilizer is pipetted up and down several times to ensure thorough mixing.

  • Q: I would like to measure the conversion of pyrophosphate to phosphate. Can I use the PiColorLock Gold Phosphate Detection System for this purpose?

    A: Yes, only the phosphate will give a signal; pyrophosphate will not.

  • Q: I have phosphate in my enzyme. What can I do?

    A: You can dialyse or desalt the enzyme into a phosphate-free buffer. Alternatively, you can use a special resin (PiBind) to remove the phosphate.

  • Q: I have 5% DMSO in my assay. Can I use PiColorLock Gold?

    A: Yes, the reagent is designed for drug screening work and other situations that require DMSO.

  • Q: I have a high background in my ATPase assay and I definitely do not have free phosphate in my sample

    A: This is almost always due to inadequate mixing of the special stabilizer with the sample and detection reagent. Make sure the stabilizer is pipetted up and down several times to ensure thorough mixing.

  • Q: I would like to measure the conversion of pyrophosphate to phosphate. Can I use the PiColorLock Gold Phosphate Detection System for this purpose?

    A: Yes, only the phosphate will give a signal; pyrophosphate will not.

  • Q: I have phosphate in my enzyme. What can I do?

    A: You can dialyse or desalt the enzyme into a phosphate-free buffer. Alternatively, you can use a special resin (PiBind) to remove the phosphate.

  • Q: I have 5% DMSO in my assay. Can I use PiColorLock Gold?

    A: Yes, the reagent is designed for drug screening work and other situations that require DMSO.

  • Q: I have a high background in my ATPase assay and I definitely do not have free phosphate in my sample

    A: This is almost always due to inadequate mixing of the special stabilizer with the sample and detection reagent. Make sure the stabilizer is pipetted up and down several times to ensure thorough mixing.

  • Q: I would like to measure the conversion of pyrophosphate to phosphate. Can I use the PiColorLock Gold Phosphate Detection System for this purpose?

    A: Yes, only the phosphate will give a signal; pyrophosphate will not.

  • Q: I have phosphate in my enzyme. What can I do?

    A: You can dialyse or desalt the enzyme into a phosphate-free buffer. Alternatively, you can use a special resin (PiBind) to remove the phosphate.

  • Q: I have 5% DMSO in my assay. Can I use PiColorLock Gold?

    A: Yes, the reagent is designed for drug screening work and other situations that require DMSO.

  • Q: I have a high background in my ATPase assay and I definitely do not have free phosphate in my sample

    A: This is almost always due to inadequate mixing of the special stabilizer with the sample and detection reagent. Make sure the stabilizer is pipetted up and down several times to ensure thorough mixing.

  • Q: I would like to measure the conversion of pyrophosphate to phosphate. Can I use the PiColorLock Gold Phosphate Detection System for this purpose?

    A: Yes, only the phosphate will give a signal; pyrophosphate will not.

Showing  1 - 4 of 4 FAQs Showing All
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