Aurora A Antibody (35C1) - BSA Free

Novus Biologicals | Catalog # NB100-1641

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Bovine

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 35C1

Format

BSA Free
View all Aurora A Primary Antibodies »

Product Specifications

Immunogen

Recombinant Aurora-A

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Bovine reactivity reported in scientific literature (PMID: 26168150)

Marker

Mitosis Marker

Specificity

Recognizes human Aurora-A kinase, a ~46kDa serine/threonine kinase involved in mitotic spindle assembly. Clone 35C1 specifically recognises an epitope within the non-catalytic N-terminal domain of Aurora-A. Clone 35C1 does not inhibit Aurora-A kinase activity.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aurora A Antibody (35C1) - BSA Free

Western Blot: Aurora A Antibody (35C1)BSA Free [NB100-1641]

Western Blot: Aurora A Antibody (35C1)BSA Free [NB100-1641]

Western Blot: Aurora A Antibody (35C1) [NB100-1641] - Analysis using the Biotin conjugate of NB100-1641. Detection of recombinant Aurora-A kinase protein probed with 100-1829 and F(ab')2 rabbit anti-mouse IgG:HRP.

Applications for Aurora A Antibody (35C1) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:10-1:500

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aurora A

NB 100-1641 recognizes human Aurora-A kinase, a 46kDa serine-threonine kinase involved in mitotic spindle assembly. Aurora-A is reported to be overexpressed in many epitheial cancers and is thought to play an important for in tumorgenesis.

Alternate Names

AIK, ARK1, AURA, AURKA, BTAK, STK15, STK6, STK7

Gene Symbol

AURKA

UniProt

O14965

Additional Aurora A Products

Product Documents for Aurora A Antibody (35C1) - BSA Free

Certificate of Analysis

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Product Specific Notices for Aurora A Antibody (35C1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Aurora A Antibody (35C1) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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