Aurora A/B/C Antibody (RM454)
Novus Biologicals | Catalog # NBP3-18823
Recombinant Monoclonal Antibody
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM454 expressed in HEK293
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Product Specifications
Immunogen
A phospho-peptide corresponding to Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (Uniprot # O14965, Q96GD4, Q9UQB9)
Specificity
This antibody reacts to human Aurora A/B/C only when phosphorylated at either Thr288, Thr232 or Thr198 respectively. There is no cross-reactivity to Aurora A/B/C that is not phosphorylated.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Aurora A/B/C Antibody (RM454)
Western Blot: Aurora A/B/C Antibody (RM454)[NBP3-18823]
Western Blot: Aurora A/B/C Antibody (RM454)[NBP3-18823]Western Blot of the lysates of HeLa cells nontreated (-) or treated (+) with Nocodazole and Calyculin A, using Anti-Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) rabbit monoclonal antibody (clone RM454) at a 1:2500 dilution.Immunohistochemistry-Paraffin: Aurora A/B/C Antibody (RM454) [NBP3-18823] -
Immunohistochemistry-Paraffin: Aurora A/B/C Antibody (RM454) [NBP3-18823] - Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section, using clone RM454 at a 1:50 dilution.Applications for Aurora A/B/C Antibody (RM454)
Application
Recommended Usage
Western Blot
1:1000-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Aurora A/B/C
Alternate Names
AIE2, AIK, AIK2, AIK3, AIM1, AIM-1, ARK1, ARK2, ARK-2, ARK3, ARK-3, AURA, AurB, AurC, aurkb-sv1, aurkb-sv2, aurora 2, aurora 3, aurora- and Ipl1-like midbody-associated protein 1, aurora kinase A, aurora kinase B, aurora kinase B-Sv1, aurora kinase B-Sv2, aurora kinase C, aurora/IPL1/EG2 protein 2, aurora/IPL1-like kinase, aurora/IPL1-related kinase 1, aurora/IPL1-related kinase 2, aurora/IPL1-related kinase 3, aurora-1, aurora-B, aurora-C, aurora-related kinase 2, aurora-related kinase 3, breast tumor-amplified kinase, BTAK, epididymis secretory protein Li 90, HEL-S-90, IPL1, PPP1R47, PPP1R48, protein phosphatase 1, regulatory subunit 47, protein phosphatase 1, regulatory subunit 48, serine/threonine kinase 12, serine/threonine kinase 13 (aurora/IPL1-like), serine/threonine protein kinase 15, serine/threonine-protein kinase 12, serine/threonine-protein kinase 13, serine/threonine-protein kinase 5, serine/threonine-protein kinase 6, serine/threonine-protein kinase aurora-A, serine/threonine-protein kinase aurora-B, serine/threonine-protein kinase aurora-C, SPGF5, STK-1, STK12, STK13, STK15, STK5, STK6, STK7
Additional Aurora A/B/C Products
Product Documents for Aurora A/B/C Antibody (RM454)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Aurora A/B/C Antibody (RM454)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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