Aurora B Antibody (3C4V8)

Novus Biologicals | Catalog # NBP3-15762

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3C4V8 expressed in HEK293
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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Aurora B (NP_004208.2). MAQKENSYPWPYGRQTAPSGLSTLPQRVLRKEPVTPSALVLMSRSNVQPTAAPGQKVMENSSGTPDILTRHFTIDDFEIGRPLGKGKFGNVYLAREKKSH

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Aurora B Antibody (3C4V8)

Western Blot: Aurora B Antibody (3C4V8) [NBP3-15762]

Western Blot: Aurora B Antibody (3C4V8) [NBP3-15762]

Western Blot: Aurora B Antibody (1N9V5) [NBP3-15762] - Western blot analysis of extracts of various cell lines, using Aurora B antibody (NBP3-15762) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Aurora B Antibody (3C4V8)

Immunohistochemistry: Aurora B Antibody (3C4V8) [Aurora B] -

Immunohistochemistry: Aurora B Antibody (3C4V8) [Aurora B] - Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using Aurora B Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Aurora B Antibody (3C4V8)

Immunohistochemistry: Aurora B Antibody (3C4V8) [Aurora B] -

Immunohistochemistry: Aurora B Antibody (3C4V8) [Aurora B] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Aurora B Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Aurora B Antibody (3C4V8)

Immunocytochemistry/ Immunofluorescence: Aurora B Antibody (3C4V8) [Aurora B] -

Immunocytochemistry/ Immunofluorescence: Aurora B Antibody (3C4V8) [Aurora B] - Confocal imaging of C6 cells using Aurora B Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Applications for Aurora B Antibody (3C4V8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Aurora B

Aurora-B is a member of a novel family of serine/threonine kinases that have been identified as key regulators of the mitotic cell division process. Aurora-B is known to be involved in the regulation of centrosome function, bipolar spindle assembly and chromosome segregation. Aurora kinases are localized at the centrosomes of interphase cells, at the poles of the bipolar spindle and in the midbody of the mitotic apparatus. Substrates identified for the Aurora-B kinases include a kinesin-like motor protein, spindle apparatus proteins, histone H3 protein, kinetochore protein and the tumor suppressor protein p53 (1). Overexpression of aurora B produced multinuclearity and induced aggressive metastasis, suggesting that overexpressed aurora B has multiple functions in cancer development. Identification of Aurora kinases as RasGAP Src homology 3 domain binding protein, also implicates these kinases as potential effectors in the Ras pathway relevant to oncogenesis (2).

Alternate Names

Aik2, AIM-1, AIM1, ARK2, AURKB, IPL1, STK12, STK5

Gene Symbol

AURKB

Additional Aurora B Products

Product Documents for Aurora B Antibody (3C4V8)

Certificate of Analysis

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Product Specific Notices for Aurora B Antibody (3C4V8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle
Epigenetics
Microtubules

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Protocols

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