Aurora B Antibody (AURKB/3121R)

Novus Biologicals | Catalog # NBP3-07554

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # AURKB/3121R
View all Aurora B Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 89-251) of human Aurora B protein (exact sequence is proprietary) (Uniprot: Q96GD4)

Localization

Nuclear and Cytoplasmic

Marker

Proliferation Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08280)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Aurora B Antibody (AURKB/3121R)

Immunohistochemistry-Paraffin: Aurora B Antibody (AURKB/3121R) [NBP3-07554]

Immunohistochemistry-Paraffin: Aurora B Antibody (AURKB/3121R) [NBP3-07554]

Immunohistochemistry-Paraffin: Aurora B Antibody (AURKB/3121R) [NBP3-07554] - Formalin-fixed, paraffin-embedded human Colon stained with Aurora B Rabbit Recombinant Monoclonal Antibody (AURKB/3121R).

Applications for Aurora B Antibody (AURKB/3121R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Aurora B

Aurora-B is a member of a novel family of serine/threonine kinases that have been identified as key regulators of the mitotic cell division process. Aurora-B is known to be involved in the regulation of centrosome function, bipolar spindle assembly and chromosome segregation. Aurora kinases are localized at the centrosomes of interphase cells, at the poles of the bipolar spindle and in the midbody of the mitotic apparatus. Substrates identified for the Aurora-B kinases include a kinesin-like motor protein, spindle apparatus proteins, histone H3 protein, kinetochore protein and the tumor suppressor protein p53 (1). Overexpression of aurora B produced multinuclearity and induced aggressive metastasis, suggesting that overexpressed aurora B has multiple functions in cancer development. Identification of Aurora kinases as RasGAP Src homology 3 domain binding protein, also implicates these kinases as potential effectors in the Ras pathway relevant to oncogenesis (2).

Alternate Names

Aik2, AIM-1, AIM1, ARK2, AURKB, IPL1, STK12, STK5

Gene Symbol

AURKB

UniProt

Q96GD4

Additional Aurora B Products

Product Documents for Aurora B Antibody (AURKB/3121R)

Certificate of Analysis

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Product Specific Notices for Aurora B Antibody (AURKB/3121R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle
Epigenetics
Microtubules

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Protocols

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