Aurora B Antibody (AURKB/3121R) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-08280

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # AURKB/3121R

Format

Azide and BSA Free
View all Aurora B Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 89-251) of human Aurora B protein (exact sequence is proprietary) (Uniprot: Q96GD4)

Localization

Nuclear and Cytoplasmic

Marker

Proliferation Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07554).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Aurora B Antibody (AURKB/3121R) - Azide and BSA Free

Immunohistochemistry-Paraffin: Aurora B Antibody (AURKB/3121R) - Azide and BSA Free [NBP3-08280]

Immunohistochemistry-Paraffin: Aurora B Antibody (AURKB/3121R) - Azide and BSA Free [NBP3-08280]

Immunohistochemistry-Paraffin: Aurora B Antibody (AURKB/3121R) - Azide and BSA Free [NBP3-08280] - Formalin-fixed, paraffin-embedded human Colon stained with Aurora B Rabbit Recombinant Monoclonal Antibody (AURKB/3121R).

Applications for Aurora B Antibody (AURKB/3121R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Aurora B

Aurora-B is a member of a novel family of serine/threonine kinases that have been identified as key regulators of the mitotic cell division process. Aurora-B is known to be involved in the regulation of centrosome function, bipolar spindle assembly and chromosome segregation. Aurora kinases are localized at the centrosomes of interphase cells, at the poles of the bipolar spindle and in the midbody of the mitotic apparatus. Substrates identified for the Aurora-B kinases include a kinesin-like motor protein, spindle apparatus proteins, histone H3 protein, kinetochore protein and the tumor suppressor protein p53 (1). Overexpression of aurora B produced multinuclearity and induced aggressive metastasis, suggesting that overexpressed aurora B has multiple functions in cancer development. Identification of Aurora kinases as RasGAP Src homology 3 domain binding protein, also implicates these kinases as potential effectors in the Ras pathway relevant to oncogenesis (2).

Alternate Names

Aik2, AIM-1, AIM1, ARK2, AURKB, IPL1, STK12, STK5

Gene Symbol

AURKB

Additional Aurora B Products

Product Documents for Aurora B Antibody (AURKB/3121R) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Aurora B Antibody (AURKB/3121R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle
Epigenetics
Microtubules

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Protocols

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FAQs

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