Autophagy/Cytotoxicity Dual Staining Kit

Novus Biologicals | Catalog # KA1299

Novus Biologicals
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Key Product Details

Species

Insect

Applications

Flow Cytometry, Functional Assay, Immunocytochemistry/ Immunofluorescence

Kit Type

Kit

Product Summary for Autophagy/Cytotoxicity Dual Staining Kit

Autophagy/Cytotoxicity Dual Staining Kit is a fluorescence-labeled method for screening autophagy and cytotoxicity at the cellular level.
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Product Specifications

Sample Volume

5 x 10^4 cells per well. All Treatments conditions should be assayed in duplicate at minimum.

Reactivity Notes

Reacts with Frugiperda.

Scientific Data Images for Autophagy/Cytotoxicity Dual Staining Kit

Functional: Autophagy/Cytotoxicity Dual Staining Kit [KA1299]

Functional: Autophagy/Cytotoxicity Dual Staining Kit [KA1299]

Functional: Autophagy/Cytotoxicity Dual Staining Other Kit [KA1299] - Tamoxifen increases autophagy but not cell death in HepG2 cells as measured by fluorescence microscopy. Panel A : MDC staining of HepG2 cells treated with vehicle. There is a basal level of autophagy, indicated by faint silver dot staining of autophagic vacuoles. Panel B : Propidium iodide staining of HepG2 cells treated with vehicle. There are few dead cells with only background staining of propidium iodide. Panel C : MDC staining of HepG2 cells treated with 10 uM Tamoxifen. Panel D : Propidium iodide staining of HepG2 cells treated with 10 uM Tamoxifen, showing a similar staining pattern to that of cells treated with vehicle

Kit Contents for Autophagy/Cytotoxicity Dual Staining Kit

  • Cell-Based Assay Buffer Tablet
  • Cell-Based Monodansylcadaverine
  • Cell-Based Propidium Iodide Solution
  • Cell-Based Tamoxifen (100 mM)

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Storage of components varies. See protocol for specific instructions.

Product Documents for Autophagy/Cytotoxicity Dual Staining Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Autophagy/Cytotoxicity Dual Staining Kit

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Citations for Autophagy/Cytotoxicity Dual Staining Kit

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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