Key Product Details

Species Reactivity

Chicken

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Avidin Primary Antibodies »

Product Specifications

Immunogen

Avidin (Hen Egg White)

Reactivity Notes

No cross reactivity occurs against Streptavidin

Specificity

No cross reactivity occurs against Streptavidin.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Avidin [Hen Egg White]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Avidin Antibody - BSA Free

Western Blot: Avidin Antibody [NB120-6675]

Western Blot: Avidin Antibody [NB120-6675]

Western Blot: Avidin Antibody [NB120-6675] - Lane 1: Avidin. Lane 2: None. Load: 100 ng per lane. Used at a dilution of 1:1000 for 1H at RT. Analysis using the Biotin conjugated antibody.
Avidin Antibody

Avidin Antibody

Western blot of Avidin Antibody. Lane 1: Molecular Weight Marker. Lane 2: Avidin. Lane 3: Streptavidin. Primary Antibody: Anti-Avidin at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP

Applications for Avidin Antibody - BSA Free

Application
Recommended Usage

ELISA

1:2000-1:20000

Immunohistochemistry

1:1000-1:5000

Immunohistochemistry-Paraffin

1:1000-1:5000

Western Blot

1:1000-1:10000
Application Notes
This product has been tested by western blot and is suitable for ELISA and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 21321260)

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Avidin

The biological function of avidin is not known. It is a homotetrameric glycoprotein found in chicken egg white, and forms a strong non covalent specific complex with biotin (one molecule of biotin per subunit of avidin).

Alternate Names

AVD, LOC396260

UniProt

P02701 (Chicken)

Additional Avidin Products

Product Documents for Avidin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Avidin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Avidin Antibody - BSA Free

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Protocols

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FAQs

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