B7-1/CD80 Antibody (2D10.4) - BSA Free

Name: B7-1/CD80 Antibody (2D10.4) - BSA Free
Brand: Novus Biologicals
SKU: NBP1-43384
Price: 419 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-43384

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Citations (2)

Product Documents

Clone 2D10.4 was used by HLDA to establish CD designation.

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Functional

Cited:

Flow Cytometry, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 2D10.4

Format

BSA Free

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Product Specifications

Immunogen

The immunogen for this antibody was CD80.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Scientific Data Images for B7-1/CD80 Antibody (2D10.4) - BSA Free

Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384]

Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384] - Analysis using the PE/Cy5 conjugate of NBP1-43384. Staining of normal human peripheral blood cells with PE-Cy5 Mouse IgG1 kappa Isotype Control (open histogram) orAnti-Human CD80 (B7-1) PE-Cy5 (filled histogram).

Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384]

Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384] - Analysis using the FITC conjugate of NBP1-43384. Staining of the Daudi cell line with Mouse IgG1 kappa Isotype Control FITC (cat. 11-4714) (blue histogram) or Anti-Human CD80 (B7-1) FITC (purple histogram). Total viable cells were used for analysis.

Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384]

Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384] - Analysis using the PE conjugate of NBP1-43384. Analysis of human peripheral blood leukocytes using CD80 PE conjugage. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8 cells/test.

Applications for B7-1/CD80 Antibody (2D10.4) - BSA Free

Application

Recommended Usage

Flow Cytometry

1:10-1:1000

Application Notes

The 2D10.4 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. Use in functional reported in scientific literature (PMID 15951837)

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Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: B7-1/CD80

The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily. CD80 is expressed by activated B cells, macrophages and dendritic cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.

Alternate Names

B71, CD80

Gene Symbol

CD80

Additional B7-1/CD80 Products

Product Documents for B7-1/CD80 Antibody (2D10.4) - BSA Free

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for B7-1/CD80 Antibody (2D10.4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.