B7-1/CD80 Antibody (8-E5-R)

Name: B7-1/CD80 Antibody (8-E5-R)
Brand: Novus Biologicals
SKU: NBP3-32169
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-32169

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG₁ Clone # 8-E5-R expressed in HEK293

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Product Specifications

Immunogen

Synthetic peptide within human B7-1/CD80 aa 74-118 / 288. (Uniprot: P33681)

Localization

Membrane.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse B7-1/CD80 Antibody (8-E5-R) (NBP3-32169) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for B7-1/CD80 Antibody (8-E5-R)

Western Blot: B7-1/CD80 Antibody (8-E5-R) [NBP3-32169]

Western Blot: B7-1/CD80 Antibody (8-E5-R) [NBP3-32169] - Western blot analysis of B7-1/CD80 on human brain tissue lysates with Mouse anti-B7-1/CD80 antibody (NBP3-32169) at 1/1,000 dilution.

Lysates/proteins at 40 ug/Lane.

Predicted band size: 33 kDa
Observed band size: 60 kDa

Exposure time: 24 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32169) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for B7-1/CD80 Antibody (8-E5-R)

Application

Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: B7-1/CD80

The B-lymphocyte activation antigen B7-1 (CD80) provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. After engagement of T-cell receptor with antigen in association with major histocompatibility complex class II, a second signal mediated through the binding of B7 to CD28 greatly upregulates the production of multiple lymphokines (1). The relatively small CTLA-4/B7-1 binding interface exhibits an unusually high degree of shape complementarity. CTLA-4 forms homodimers through a newly defined interface of highly conserved residues. This zipper-like oligomerization provides the structural basis for forming unusually stable signalling complexes at the T-cell surface, underscoring the importance of potent inhibitory signalling in human immune responses (2).

Alternate Names

B71, CD80

Gene Symbol

CD80

Additional B7-1/CD80 Products

Product Documents for B7-1/CD80 Antibody (8-E5-R)

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Product Specific Notices for B7-1/CD80 Antibody (8-E5-R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.