B7-H3/CD276 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-32251
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP2-32251]](https://resources.rndsystems.com/images/products/B7-H3-CD276-Antibody-Immunohistochemistry-NBP2-32251-img0002.jpg "Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP2-32251]")
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residue 484 to 534 of human CD276 Molecule using the numbering given in entry NP_001019907.1 (GeneID 80381).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for B7-H3/CD276 Antibody - BSA Free
Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP2-32251]
Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP2-32251] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-B7-H3/CD276 (NBP2-32251). Detection: DAB Counterstain: IHC hematoxylin![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP2-32251]](https://resources.rndsystems.com/images/products/B7-H3-CD276-Antibody-Immunocytochemistry-NBP2-32251-img0003.jpg "Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP2-32251]")
Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP2-32251]
Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP2-32251] - Samples: FFPE sections of MCF-7 cells (endogenous expression) and CD276 transfected Hek293 cells (over-expression). Antibody: Affinity purified rabbit anti-B7-H3/CD276 (NBP2-32251). Detection: DAB![Immunoprecipitation: B7-H3/CD276 Antibody [NBP2-32251]](https://resources.rndsystems.com/images/products/B7-H3-CD276-Antibody-Immunoprecipitation-NBP2-32251-img0001.jpg "Immunoprecipitation: B7-H3/CD276 Antibody [NBP2-32251]")
Immunoprecipitation: B7-H3/CD276 Antibody [NBP2-32251]
Immunoprecipitation: B7-H3/CD276 Antibody [NBP2-32251] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-CD276 antibody NBP2-32251 used for IP at 6 ug per reaction. CD276 was also immunoprecipitated by rabbit anti-CD276 antibody BL16383. For blotting immunoprecipitated CD276 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for B7-H3/CD276 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:2000 - 1:10000
Immunohistochemistry-Paraffin
1:2000 - 1:10000
Immunoprecipitation
2 - 10 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: B7-H3
Additional B7-H3 Products
Product Documents for B7-H3/CD276 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for B7-H3/CD276 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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