B7-H6 Antibody (17B1.3) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-09049

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 kappa Clone # 17B1.3

Format

Azide and BSA Free
View all B7-H6 Primary Antibodies »

Product Specifications

Immunogen

Immunised mice with extracellular domain of human B7-H6 and selected on the basis of specific reactivity by P815.B7-H6, but not with P815.B7-H1.

Specificity

mAb 17B1.3 specifically recognises B7-H6. 17B1.3 reacted with human tumour cell lines constitutively expressing B7-H6 such as HeLa cells. Fab 17B1.3 does not interfere with the binding of B7-H6 to NKp30, but nevertheless blocks NK cell activation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for B7-H6 Antibody (17B1.3) - Azide and BSA Free

Flow Cytometry: B7-H6 Antibody (17B1.3) - Azide and BSA Free [NBP3-09049]

Flow Cytometry: B7-H6 Antibody (17B1.3) - Azide and BSA Free [NBP3-09049]

Flow Cytometry: B7-H6 Antibody (17B1.3) [NBP3-09049] - HEK293T cells were harvested using PBS/1 mM EDTA and stained with B7-H6 antibody and/or secondary antibody. Image from verified customer review.

Applications for B7-H6 Antibody (17B1.3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
B7-H6 is not detected in normal human tissues at steady state but is expressed on tumour cells, so tumour cells can be detected by NK cells. This article describes the study of the mechanisms that govern the induction of B7-H6 on primary cells as well as its consequences in vitro and in vivo.

Reviewed Applications

Read 1 review rated 5 using NBP3-09049 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: B7-H6

B7-H6 is a transmembrane immune regulatory protein that binds to NKp30 expressed on NK cells. Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis. B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells. The expression of NKp30 ligands on tumor cells correlates with tumor cell sensitivity to NKp30-dependent cell lysis.

Long Name

B7 Homolog 6

Alternate Names

B7H6, DKFZp686I21167, DKFZp686O24166, NCR3LG1

Gene Symbol

NCR3LG1

Additional B7-H6 Products

Product Documents for B7-H6 Antibody (17B1.3) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for B7-H6 Antibody (17B1.3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for B7-H6 Antibody (17B1.3) - Azide and BSA Free (1)

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Customer Images

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  • B7-H6 Antibody (17B1.3)
    Name: Hajime Asada
    Application: Flow Cytometry
    Sample Tested: HEK293T cells
    Species: Human
    Verified Customer | Posted 10/06/2022
    Blue: 17B1.3 and Alexa-Fluor 488 conjugated secondary antibody. Red: Only secondary antibody.
    HEK293T cells were harvested using PBS/1 mM EDTA and stained with 17B1.3 and/or secondary antibody.
    B7-H6 Antibody (17B1.3) - Azide and BSA Free NBP3-09049

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