B cell-activating factor receptor (BAFFR) is a type III transmembrane protein belonging to the TNF receptor superfamily (TNFRSF13C). It is one of three TNFRSF members that binds to, and is activated by, BAFF. BAFFR binds only BAFF and no other TNFSF ligands. It is the critical receptor for BAFF during Blymphopoiesis.
BAFFR/TNFRSF13C Antibody (11C1) - BSA Free
Novus Biologicals | Catalog # NBP3-09142
Clone 11C1 was used by HLDA to establish CD designation.
![Flow Cytometry: BAFFR/TNFRSF13C Antibody (11C1) - BSA Free [NBP3-09142]](https://resources.rndsystems.com/images/products/BAFFR-TNFRSF13C-Antibody-11C1-Flow-Cytometry-NBP3-09142-img0002.jpg "Flow Cytometry: BAFFR/TNFRSF13C Antibody (11C1) - BSA Free [NBP3-09142]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 11C1
Format
BSA Free
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Product Specifications
Immunogen
human BAFFR/TNFRSF13C-transfected murine L1.2 cells
Specificity
The mouse monoclonal antibody 11C1 recognizes an extracellular epitope of CD268 / BAFF R (B cell-activating factor receptor), a 19 kDa type III transmembrane protein expressed on resting B cells and CD4-positive T cells, but down regulated after activation.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for BAFFR/TNFRSF13C Antibody (11C1) - BSA Free
Flow Cytometry: BAFFR/TNFRSF13C Antibody (11C1) - BSA Free [NBP3-09142]
Flow Cytometry: BAFFR/TNFRSF13C Antibody (11C1) [NBP3-09142] - Separation of human CD268 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD268 (11C1) purified antibody (concentration in sample 0,6 ug/ml, GAM APC).![Flow Cytometry: BAFFR/TNFRSF13C Antibody (11C1) - BSA Free [NBP3-09142]](https://resources.rndsystems.com/images/products/BAFFR-TNFRSF13C-Antibody-11C1-Flow-Cytometry-NBP3-09142-img0001.jpg "Flow Cytometry: BAFFR/TNFRSF13C Antibody (11C1) - BSA Free [NBP3-09142]")
Flow Cytometry: BAFFR/TNFRSF13C Antibody (11C1) - BSA Free [NBP3-09142]
Flow Cytometry: BAFFR/TNFRSF13C Antibody (11C1) [NBP3-09142] - Surface staining pattern of human peripheral whole blood stained using anti-human CD268 (11C1) purified antibody (concentration in sample 0,6 ug/ml, GAM APC).Applications for BAFFR/TNFRSF13C Antibody (11C1) - BSA Free
Application
Recommended Usage
Flow Cytometry
1-4 ug/ml
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
Phosphate buffered saline (PBS), pH 7.4
Format
BSA Free
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BAFFR/TNFRSF13C
Long Name
B cell Activating Factor Receptor
Alternate Names
BAFF R, BR3, CD268, TNFRSF13C
Gene Symbol
TNFRSF13C
Additional BAFFR/TNFRSF13C Products
Product Documents for BAFFR/TNFRSF13C Antibody (11C1) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for BAFFR/TNFRSF13C Antibody (11C1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
