Bag-1 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56082

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Bag-1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 26-45 (NERYDLLVTPQQGNSEPVVQD) of mouse BAG-1 was used as immunogen; GenBank no. AAH03722.1. This sequence is 100% conserved in mouse BAG-1L; GenBank no. NP_033866.3.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Bag-1 Antibody - BSA Free

Western Blot: Bag-1 Antibody [NB100-56082]

Western Blot: Bag-1 Antibody [NB100-56082]

Western Blot: Bag-1 Antibody [NB100-56082] - Analysis of BAG-1 in normal brain tissue lysates.
Immunohistochemistry-Paraffin: Bag-1 Antibody [NB100-56082]

Immunohistochemistry-Paraffin: Bag-1 Antibody [NB100-56082]

Immunohistochemistry-Paraffin: Bag-1 Antibody [NB100-56082] - Formalin-fixed, paraffin-embedded human breast tissue stained for BAG-1 expression using this antibody at 1:2000. A. normal breast epithelium. B. breast cancer. A1 and B1 are high magnifications from A and B, respectively. Hematoxylin-eosin counterstain.
Western Blot: Bag-1 Antibody [NB100-56082]

Western Blot: Bag-1 Antibody [NB100-56082]

Western Blot: Bag-1 Antibody [NB100-56082] - BAG-1 levels in human neuroblastoma. Image from verified customer review.
Immunohistochemistry-Paraffin: Bag-1 Antibody [NB100-56082]

Immunohistochemistry-Paraffin: Bag-1 Antibody [NB100-56082]

Immunohistochemistry-Paraffin: Bag-1 Antibody [NB100-56082] - Formalin-fixed, paraffin-embedded mouse ischemic brain stained for BAG-1 expression using NB100-56082 at 1:2000. A1 is a higher magnification of A. Differential Bag-1 expression levels are seen in the hypothalmic nuclei. The variation in expression levels from lack of Bag-1 staining to strong staining may be linked to differential responses of the hypothalmic nuclei to ischemic stress. Hematoxylin-eosin counterstain.

Applications for Bag-1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:50-1:200

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bag-1

The BAG (Bcl-2-associated anthanogene) proteins are a family of chaperone regulators that modulate a number of diverse processes including proliferation, survival, stress responses, tumorigenesis, neuronal differentiation, growth arrest and apoptosis (reviewed Takayama and Reed, 2001; Doong et al, 2002, and Doukhanina et al. 2006). BAG proteins have been characterized as co-chaperones and interact with the chaperone heat shock proteins 70, both constitutive Hsc70 and inducible Hsp70. BAG proteins bind through their BAG domain to the ATPase domain of Hsc70/Hsp70, and can modulate either positively or negatively the functions of the Hsc70/Hsp70 chaperone proteins. The BAG domain has been shown to contribute to the anti-apoptotic activity of BAG- family proteins. The anti-apoptotic activities of BAG-family proteins may be dependent on their interactions with Hsc70/Asp70 and/or binding to Bcl-2. In addition to the conserved BAG domain, BAG-family proteins also contain additional domains which enable them to interact with specific target proteins or to target them to specific locations within cells. The BAG family contains at least six family members, including BAG-1 and its various isoforms [including BAG-1S, BAG-1M (RAP46/HAP46), and BAG-1L, BAG2, BAG3 (CAIR-1; Bis,), BAG4 (SODD), BAG5 and BAG6 (Scythe, BAT3). The following amino acids (aa) lengths and molecular weights (kDa) have been described for human BAG proteins (reviewed in Takayama et al, 2001 and Doong et al, 2002): BAG-1 (230 aa., 34 kDa), BAG-1S (219 aa, 29 kDa), BAG-1M (274 aa, 46 kDa), BAG-1L (345 aa, 52 kDa), BAG-2 [212 aa; 24 kDa (Arndt et al. 2005)], BAG-3 (575 aa, 74 kDa), BAG-4 (456 aa; 60 kDa), BAG-5 ([442 aa; 51 kDa (Kalia et al. 2004)], and BAG-6 (1129 aa; 150 kDa). NB100-56082 recognizes both BAG-1 and BAG-1 isoforms which contain the peptide immunogen sequence, including BAG-1L. Mouse BAG-1 is a 219 amino acid (aa) protein (GenBank no. AAH03722.1) and mouse BAG-1L is a 355 aa protein (AAH03722.1)

Long Name

Bcl-2-associated Anthanogene-1

Alternate Names

Bag1

Entrez Gene IDs

573 (Human); 12017 (Mouse); 297994 (Rat)

Gene Symbol

BAG1

Additional Bag-1 Products

Product Documents for Bag-1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Bag-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for Bag-1 Antibody - BSA Free

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Protocols

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FAQs

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