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Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 5A8
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Product Specifications
Immunogen
BAG3 (AAH14656.1, 1 a.a. ~ 575 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSAATHSPMMQVASGNGDRDPLPPGWEIKIDPQTGWPFFVDHNSRTTTWNDPRVPSEGPKETPSSANGPSREGSRLPPAREGHPVYPQLRPGYIPIPVLHEGAENRQVHPFHVYPQPGMQRFRTEAAAAAPQRSQSPLRGMPETTQPDKQCGQVAAAAAAQPPASHGPERSQSPAASDCSSSSSSASLPSSGRSSLGSHQLPRGYISIPVIHEQNVTRPAAQPSFHQAQKTHYPAQQGEYQTHQPVYHKIQGDDWEPRPLRAASPFRSSVQGASSREGSPARSSTPLHSPSPIRVHTVVDRPQQPMTHRETAPVSQPENKPESKPGPVGPELPPGHIPIQVIRKEVDSKPVSQKPPPPSEKVEVKVPPAPVPCPPPSPGPSAVPSSPKSVATEERAAPSTAPAEATPPKPGEAEAPPKHPGVLKVEAILEKVQGLEQAVDNFEGKKTDKKYLMIEEYLTKELLALDSVDPEGRADVRQARRDGVRKVQTILEKLEQKAIDVPGQVQVYELQPSNLEADQPLQAIMEMGAVAADKGKKNAGNAEDPHTETQQPEATAAATSNPSSMTDTPGNPAAP
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for BAG3 Antibody (5A8)
Western Blot: BAG3 Antibody (5A8) [H00009531-M01A]
Western Blot: BAG3 Antibody (5A8) [H00009531-M01A] - Analysis of BAG3 expression in transfected 293T cell line by BAG3 monoclonal antibody (M01A), clone 5A8.Lane 1: BAG3 transfected lysate (Predicted MW: 61.6 KDa).Lane 2: Non-transfected lysate.Applications for BAG3 Antibody (5A8)
Application
Recommended Usage
ELISA
1:100 - 1:2000
Western Blot
1:100 - 1:2000
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Ascites
Preservative
No Preservative
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: BAG3
Alternate Names
BAG family molecular chaperone regulator 3, BAG-3, BAG-family molecular chaperone regulator-3, Bcl-2-associated athanogene 3, BCL2-associated athanogene 3, BCL2-binding athanogene 3, Bcl-2-binding protein Bis, BIS, CAIR-1, Docking protein CAIR-1, MGC104307
Gene Symbol
BAG3
Additional BAG3 Products
Product Documents for BAG3 Antibody (5A8)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for BAG3 Antibody (5A8)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- ELISA Sample Preparation & Collection Guide
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- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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