BAP1 Overexpression Lysate
Novus Biologicals | Catalog # NBL1-07915
![Western Blot: BAP1 Overexpression Lysate [NBL1-07915]](https://resources.rndsystems.com/images/products/BAP1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07915-img0002.jpg "Western Blot: BAP1 Overexpression Lysate [NBL1-07915]")
Key Product Details
Species
Applications
Product Summary for BAP1 Overexpression Lysate
BAP1 Transient Overexpression Lysate
Expression Host: HEK293T
Plasmid: RC200378
Accession#: NM_004656
Protein Tag: C-MYC/DDK
You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Product Specifications
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Type
Scientific Data Images for BAP1 Overexpression Lysate
Western Blot: BAP1 Overexpression Lysate [NBL1-07915]
Western Blot: BAP1 Overexpression Lysate (Adult Normal) [NBL1-07915] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BAP1.Formulation, Preparation, and Storage
Formulation
Concentration
Shipping
Storage
Background: BAP1
Long Name
Alternate Names
Gene Symbol
Additional BAP1 Products
Product Documents for BAP1 Overexpression Lysate
Certificate of Analysis
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Product Specific Notices for BAP1 Overexpression Lysate
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for BAP1 Overexpression Lysate
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Q: I just received your BAP1 Lysate. Couple of questions: 1 - Is it already in RIPA buffer? 2- If so what is the additional vial of RIPA buffer for? 3 - What is the volume of lysate in the vial? 4 - How much do you load in your gel?
A: The lysate is in RIPA bufer. The contents should be: 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer and 1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer and 1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT). The additional vial should be the empty vector control in RIPA. This is the vector without the gene cloned into it. The volume is 100ul. Use 10 ug/lane.
Associated Pathways
