BARHL1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-86513
![Immunohistochemistry-Paraffin: BARHL1 Antibody [NBP1-86513]](https://resources.rndsystems.com/images/products/BARHL1-Antibody-Immunohistochemistry-Paraffin-NBP1-86513-img0013.jpg "Immunohistochemistry-Paraffin: BARHL1 Antibody [NBP1-86513]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse, Rat
Predicted:
Rat (96%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Chromatin Immunoprecipitation (ChIP)
Cited:
Immunocytochemistry/ Immunofluorescence, IF/IHC, Protein Array
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LELSPRSESSSDCSSPASPGRDCLETGTPRPGGASGPGLDSHLQPGQLSAPAQSRTVTSSFLIRDILADCKPLAACAPYSSSGQPAAPEPGGRLAAKAAEDFRDKLDKSGSNASSDSEYKVKEEGDREISSSRDSP
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BARHL1 Antibody - BSA Free
Immunohistochemistry-Paraffin: BARHL1 Antibody [NBP1-86513]
Immunohistochemistry-Paraffin: BARHL1 Antibody [NBP1-86513] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.![Immunohistochemistry-Paraffin: BARHL1 Antibody [NBP1-86513]](https://resources.rndsystems.com/images/products/BARHL1-Antibody-Immunohistochemistry-Paraffin-NBP1-86513-img0009.jpg "Immunohistochemistry-Paraffin: BARHL1 Antibody [NBP1-86513]")
Immunohistochemistry-Paraffin: BARHL1 Antibody [NBP1-86513]
Immunohistochemistry-Paraffin: BARHL1 Antibody [NBP1-86513] - Staining of mouse embryo E14 shows strong nuclear positivity in neuronal cells in developing brain stem and cerebellum.![Immunohistochemistry-Paraffin: BARHL1 Antibody [NBP1-86513]](https://resources.rndsystems.com/images/products/BARHL1-Antibody-Immunohistochemistry-Paraffin-NBP1-86513-img0011.jpg "Immunohistochemistry-Paraffin: BARHL1 Antibody [NBP1-86513]")
Immunohistochemistry-Paraffin: BARHL1 Antibody [NBP1-86513]
Immunohistochemistry-Paraffin: BARHL1 Antibody [NBP1-86513] - Staining of human cerebellum shows weak nuclear positivity in cells in molecular layer.![Immunohistochemistry-Paraffin: BARHL1 Antibody [NBP1-86513]](https://resources.rndsystems.com/images/products/BARHL1-Antibody-Immunohistochemistry-Paraffin-NBP1-86513-img0012.jpg "Immunohistochemistry-Paraffin: BARHL1 Antibody [NBP1-86513]")
Immunohistochemistry-Paraffin: BARHL1 Antibody [NBP1-86513]
Immunohistochemistry-Paraffin: BARHL1 Antibody [NBP1-86513] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Chromatin Immunoprecipitation ChIP: BARHL1 Antibody - BSA Free
ChIP-Exo-Seq composite graph for Anti-BARHL1 tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh's Lab at Cornell University.Applications for BARHL1 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1-10µg per reaction
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. Please note that ICC/IF was reported in PMID 31641054, which used a previous lot that is no longer available. The current lot of this antibody is not validated for use in ICC/IF. Please contact technical services if you have any questions.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BARHL1
Alternate Names
BarH (Drosophila)-like 1, barH-like 1 homeobox protein, BarH-like homeobox 1
Gene Symbol
BARHL1
Additional BARHL1 Products
Product Documents for BARHL1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for BARHL1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for BARHL1 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ChIP Protocol Video
- Chromatin Immunoprecipitation (ChIP) Protocol
- Chromatin Immunoprecipitation Protocol
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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