Bassoon Antibody

Novus Biologicals | Catalog # NBP1-46351

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Bassoon Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from internal region of human Bassoon conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Bassoon Antibody

Bassoon Antibody

Immunohistochemistry: Bassoon Antibody [NBP1-46351] -

AICP treatment immediately after fear extinction increases synaptic alpha -amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors. (A) Confocal images from basolateral amygdala (BLA) of vehicle-extinction (top) and AICP extinction (100 ng/side, i-BLA, bottom) WT mice immunostained for AMPA receptor GluA1 subunit and PSD 95. Arrowheads showing colocalized puncta. (B) Quantification of immunostaining; AICP treatment led to a significant increase in total GluA1 puncta (P =.0196). Trend for an increase in PSD 95 immunoreactive puncta in AICP-extinction group (P =.0926). Significant increase in total GluA1-PSD 95 colocalized puncta in AICP-extinction group (P =.0002). (C) Confocal images from BLA of vehicle-extinction (top) and AICP-extinction (bottom) WT mice immunostained for bassoon, GluA1 and PSD 95. Arrowheads show colocalized puncta. (D) Quantification of immunostaining. No significant change in bassoon-PSD 95 colocalized puncta by AICP (P =.1387). Significant increase in bassoon/GluA1/PSD 95 colocalized puncta (P =.0282) by AICP treatment. Bar graphs represent mean +/- SEM, each data point representing 22–24 images from n = 3 vehicle and n = 5 AICP mice. Data were analyzed by unpaired t test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34363482), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Bassoon Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:1000

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Bassoon

Bassoon is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle (1-3). Bassoon does not belong to any known protein families. It has been found in rat and mouse with sequence identity of the two proteins at 96% (1). The human BASSOON gene has recently been cloned and localized (3). Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains (1). The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntingtin, abnormal amplification of this region can cause late-onset neurodegeneration (1,3). Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors (2). Another presynaptic protein, Piccolo (4) was found to colocalize with Bassoon in cultured hippocampal neurons (1). These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release (1).

Alternate Names

bassoon (presynaptic cytomatrix protein)

Entrez Gene IDs

8927 (Human)

Gene Symbol

BSN

Additional Bassoon Products

Product Documents for Bassoon Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Bassoon Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Bassoon Antibody

Customer Reviews for Bassoon Antibody

There are currently no reviews for this product. Be the first to review Bassoon Antibody and earn rewards!

Have you used Bassoon Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Bassoon Antibody

Showing  1 - 2 of 2 FAQs Showing All

  • Q: I am looking for a good antibody to detect Bassoon in mouse brain slices (PFA fixed); can you recommend any one for this?

    A:

    After searching through our product catalog list, we have found the following antibodies which may be of interest: Bassoo. Using the filters on the left column of the site should allow you to filter by species and desired application. Any antibody that is listed to show validation for use in IHC-P and has also been validated in mouse samples will be backed by our 100% Guarantee. Under the terms and conditions of the guarantee, Novus will provide you with a full refund or replacement should the antibody fail to perform, as specified, in samples expressing the protein.

  • Q: We are interested in Anti Bassoon Ab (NBP1-46351), but we need the exact epitope sequence.

    A: Unfortunately we cannot provide the specific immunogen sequence as it is proprietary; however I can tell you that the immunogen is found between amino acids 1000 and 1100 in the parental protein.

  • Q: I am looking for a good antibody to detect Bassoon in mouse brain slices (PFA fixed); can you recommend any one for this?

    A:

    After searching through our product catalog list, we have found the following antibodies which may be of interest: Bassoo. Using the filters on the left column of the site should allow you to filter by species and desired application. Any antibody that is listed to show validation for use in IHC-P and has also been validated in mouse samples will be backed by our 100% Guarantee. Under the terms and conditions of the guarantee, Novus will provide you with a full refund or replacement should the antibody fail to perform, as specified, in samples expressing the protein.

  • Q: We are interested in Anti Bassoon Ab (NBP1-46351), but we need the exact epitope sequence.

    A: Unfortunately we cannot provide the specific immunogen sequence as it is proprietary; however I can tell you that the immunogen is found between amino acids 1000 and 1100 in the parental protein.

Showing  1 - 2 of 2 FAQs Showing All
View all FAQs for Antibodies