BBOX1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-92462

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all BBOX1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human BBOX1 (NP_003977.1). MACTIQKAEALDGAHLMQILWYDEEESLYPAVWLRDNCPCSDCYLDSAKARKLLVEALDVNIGIKGLIFDRKKVYITWPDEHYSEFQADWLKKRCFSKQARAKLQRELFFPECQYWGSELQLPTLDFEDVLRYDEHAYKWLSTLKKVGIVRLTGASDKPGEVSKLGKRMGFLYLTFYGHT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BBOX1 Antibody - BSA Free

BBOX1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: BBOX1 Antibody - BSA Free [NBP2-92462] -

Immunocytochemistry/ Immunofluorescence: BBOX1 Antibody - BSA Free [NBP2-92462] - Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of mouse kidney cells using BBOX1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
BBOX1 Antibody - BSA Free

Western Blot: BBOX1 Antibody - BSA Free [NBP2-92462] -

Western Blot: BBOX1 Antibody - BSA Free [NBP2-92462] - Western blot analysis of extracts of various cell lines, using BBOX1 antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.

Applications for BBOX1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BBOX1

BBOX1 encodes gamma butyrobetaine hydroxylase which catalyzes the formation of L-carnitine from gamma-butyrobetaine, the last step in the L-carnitine biosynthetic pathway. Carnitine is essential for the transport of activated fatty acids across the mitochondrial membrane during mitochondrial beta-oxidation.

Alternate Names

2-oxoglutarate dioxygenase, 2-oxoglutarate dioxygenase 1, BBHgamma-butyrobetaine, BBOXEC 1.14.11.1, butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetainehydroxylase) 1, gamma-BBH, Gamma-butyrobetaine, gamma-butyrobetaine dioxygenase, Gamma-butyrobetaine hydroxylase, G-BBH

Gene Symbol

BBOX1

Additional BBOX1 Products

Product Documents for BBOX1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for BBOX1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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