BCKDHA Antibody (BLR060F)

Novus Biologicals | Catalog # NBP3-14681

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, Simple Western, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR060F
View all BCKDHA Primary Antibodies »

Product Specifications

Immunogen

surrounding serine 292

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for BCKDHA Antibody (BLR060F)

Immunoprecipitation: BCKDHA Antibody (BLR060F) [NBP3-14681]

Immunoprecipitation: BCKDHA Antibody (BLR060F) [NBP3-14681]

Immunoprecipitation: BCKDHA Antibody [NBP3-14681] - Whole cell lysate (1 mg per IP reaction, 20% of IP loaded) from Hep-G2 cells treated with phosphatase (+) or mock treated (-).Rabbit anti-BCKDHA antibody used for IP at 6 ug per reaction. For blotting immunoprecipitated rabbit anti-Phospho BCKDHA recombinant monoclonal antibody was used at 1:1000 (upper panel) was used at 1 ug/ml (lower panel). HRP-conjugated goat anti-rabbit IgG Chemiluminescence with an exposure time of 30 seconds.
BCKDHA Antibody (BLR060F)

Simple Western: BCKDHA Antibody [NBP3-14681] -

Simple Western: BCKDHA Antibody [NBP3-14681] - Detection of mouse pBCKDHA (S292) by Simple Western™. Samples: Whole cell lysate (0.4 mg/mL) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody [BLR060F] (NBP3-14681) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.
BCKDHA Antibody (BLR060F)

Simple Western: BCKDHA Antibody [NBP3-14681] -

Simple Western: BCKDHA Antibody [NBP3-14681] - Detection of human pBCKDHA (S292) by Simple Western™. Samples: Whole cell lysate (0.08 mg/mL) from HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody [BLR060F] (NBP3-14681) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.

Applications for BCKDHA Antibody (BLR060F)

Application
Recommended Usage

Simple Western

1:10 - 1:50
Application Notes
See Simple Western Antibody Database for Simple Western validation: Tested in 3T3 [0.4 mg/mL], separated by Size, antibody dilution of 1:10, 1:50, apparent MW was 52 kDa

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: BCKDHA

BCKDHA - branched chain keto acid dehydrogenase E1, alpha polypeptide (maple syrup urine disease)

Alternate Names

2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial, BCKDE1A, BCKDH E1-alpha, branched chain keto acid dehydrogenase E1, alpha polypeptide, branched chain keto acid dehydrogenase E1, alpha polypeptide (maple syrup urinedisease), Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain, EC 1.2.4.4,2-oxoisovalerate dehydrogenase (lipoamide), FLJ45695, MSU, MSUD1, OVD1A

Gene Symbol

BCKDHA

Additional BCKDHA Products

Product Documents for BCKDHA Antibody (BLR060F)

Certificate of Analysis

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Product Specific Notices for BCKDHA Antibody (BLR060F)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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