Human Bcl-2 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP1-91188

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Cell lysate, serum, plasma (20 uL)

Sensitivity

0.5 ng/mL

Assay Range

0.50 - 32.0 ng/mL
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Description

The human Bcl-2 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Bcl-2.

Precision

Intra-Assay Precision (Precision within an assay) CV% 8.60%

Inter-Assay Precision (Precision between assays) CV% 12%

Recovery for Human Bcl-2 ELISA Kit (Colorimetric)

Recovery

71%

Scientific Data Images for Human Bcl-2 ELISA Kit (Colorimetric)

ELISA: Human Bcl-2 ELISA Kit (Colorimetric) [NBP1-91188]

ELISA: Human Bcl-2 ELISA Kit (Colorimetric) [NBP1-91188]

ELISA: Bcl-2 ELISA Kit [NBP1-91188] - Standard curve

Kit Contents for Human Bcl-2 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Bcl-2
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-human Bcl-2 monoclonal antibody
  • Human Bcl-2 Standard lyophilized, 64 ng/mL upon reconstitution
  • Lysis Buffer 10x
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Bcl-2

The human Bcl-2 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Bcl-2. B-cell lymphoma 2 encoded by a proto-oncogene, is an intracellular membrane-associated protein that functions to block programmed cell death. Expression of Bcl-2 has been reported in a wide range of hematopoietic cells, nonneoplastic epithelia and epithelial malignancies. Bcl-2 levels are low or undetectable in endothelial cells. A decrease in Bcl-2 levels leads to cell death by apoptosis. Overexpression of Bcl-2 on the other hand protects cells from death, but it is neither able to immortalize normal cells, nor to cause tumorgenic transformation of immortalized cells.

Long Name

B Cell Lymphoma/Leukemia 2

Alternate Names

Bcl2

Gene Symbol

BCL2

Additional Bcl-2 Products

Product Documents for Human Bcl-2 ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human Bcl-2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Human Bcl-2 ELISA Kit (Colorimetric)

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  • Q: Which BCL-2 isoform is detected with this kit (alpha or beta)? Has any epitope mapping been performed for this kit?

    A: The immunogen regions for the 2 antibodies utilized in this Bcl2 ELISA kit are proprietary and cannot be released. This kit does recognizes both the alpha and beta isoform of the Bcl-2 protein.

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