Q: Are the beta-Actin antibodies validated in Simple Western?

A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.

Q: Do Beta-Actin antibodies come in lyophilized format?

A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.

Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

A: For homogenized tissue, beta-actin and GAPDH are both fine.

Q: What is the theoretical molecular weight for Beta-Actin antibodies?

A: The TMW for beta Actin antibodies is approximately 42 kDa.

Q: Why is beta actin used as a loading control in blotting ?

A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.

beta-Actin Antibody (CL3868) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44487

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Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # CL3868

Format

Azide and BSA Free

View all beta-Actin Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P60709, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for beta-Actin Antibody (CL3868) - Azide and BSA Free

Western Blot: beta-Actin Antibody [NBP3-44487]

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line HeLa
Lane 3: Human cell line HEK 293
Lane 4: Human cell line A-431
Lane 5: Human cell line HepG2
Lane 6: Mouse cell line NIH-3T3
Lane 7: Rat cell line NBT-II

Immunohistochemistry-Paraffin: beta-Actin Antibody [NBP3-44487]

Staining of human colon shows positivity in smooth muscle, lymphoid and glandular cells.

Immunohistochemistry-Paraffin: beta-Actin Antibody [NBP3-44487]

Staining of human fallopian tube shows immunoreactivity in smooth muscle cells of blood vessels and connective tissue.

Immunohistochemistry-Paraffin: beta-Actin Antibody [NBP3-44487]

Staining of human prostate shows strong cytoplasmic immunoreactivity in smooth muscle and weak positivity in glandular cells.

Immunohistochemistry-Paraffin: beta-Actin Antibody [NBP3-44487]

Staining of human tonsil shows strong positivity in lymphoid cells.

Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody [NBP3-44487]

Staining of U-251 cells using the Anti-beta-Actin monoclonal antibody).

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Applications for beta-Actin Antibody (CL3868) - Azide and BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-4 ug/ml

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: beta-Actin

Actin is a highly conserved protein involved in cell motility, structure, and integrity. Six functional actin isoforms exist and were named based on their electrophoretic mobility and distribution including alpha-cardiac muscle actin (aCAA), alpha-skeletal muscle actin (aSKA), alpha-smooth muscle actin (aSMA), beta-cytoplasmic actin (bCYA), gamma-cytoplasmic actin (gCYA) and gamma-smooth muscle actin (gSMA) (1). The cytoplasmic isoforms, beta-actin and gamma-actin, are nearly identical, differing by only 4 amino acids at the N-terminus with a theoretical molecular weight of 42 kDa (2). Beta-actin is ubiquitously expressed in eukaryotic cells and considered a housekeeping gene which is frequently used as a loading control for assays involving protein detection, such as Western blotting (3,4).

References

1. Vandekerckhove J, Weber K. 1978. At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4):783-802. PMID: 745245

2. Gimona M, Vandekerckhove J, Goethals M, Herzog M, Lando Z, Small JV. (1994) Beta-actin specific monoclonal antibody. Cell Motil Cytoskeleton. 27(2):108-16. PMID: 8162619

3. Holden VI, Lenio S, Kuick R, Ramakrishnan SK, Shah YM, Bachman MA. (2014) Bacterial siderophores that evade or overwhelm Lipocalin 2 induce HIF-1a and pro-inflammatory cytokine secretion in cultured respiratory epithelial cells Infect Immun. 82(9):3826-36 PMID: 24980968

4. Tsai WL, Yeh PH, Tsai CY, Ting CT, Chiu YH, Tao MH, Li WC, Hung SC. (2016) Efficient Programming of Human Mesenchymal Stem Cell Derived Hepatocytes by Epigenetic Regulations. J. Gastroenterol. Hepatol. 32(1):261-269. PMID: 27218433

Alternate Names

ACTB, betaActin

Gene Symbol

ACTB

Additional beta-Actin Products

Product Documents for beta-Actin Antibody (CL3868) - Azide and BSA Free

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for beta-Actin Antibody (CL3868) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Adhesion Molecules on Endothelial Cells

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for beta-Actin Antibody (CL3868) - Azide and BSA Free

Showing 1 - 5 of 5 FAQs Showing All

Q: Are the beta-Actin antibodies validated in Simple Western?

A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
Q: Do Beta-Actin antibodies come in lyophilized format?

A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

A: For homogenized tissue, beta-actin and GAPDH are both fine.
Q: What is the theoretical molecular weight for Beta-Actin antibodies?

A: The TMW for beta Actin antibodies is approximately 42 kDa.
Q: Why is beta actin used as a loading control in blotting ?

A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
Q: Are the beta-Actin antibodies validated in Simple Western?

A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
Q: Do Beta-Actin antibodies come in lyophilized format?

A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

A: For homogenized tissue, beta-actin and GAPDH are both fine.
Q: What is the theoretical molecular weight for Beta-Actin antibodies?

A: The TMW for beta Actin antibodies is approximately 42 kDa.
Q: Why is beta actin used as a loading control in blotting ?

A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
Q: Are the beta-Actin antibodies validated in Simple Western?

A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
Q: Do Beta-Actin antibodies come in lyophilized format?

A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

A: For homogenized tissue, beta-actin and GAPDH are both fine.
Q: What is the theoretical molecular weight for Beta-Actin antibodies?

A: The TMW for beta Actin antibodies is approximately 42 kDa.
Q: Why is beta actin used as a loading control in blotting ?

A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
Q: Are the beta-Actin antibodies validated in Simple Western?

A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
Q: Do Beta-Actin antibodies come in lyophilized format?

A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

A: For homogenized tissue, beta-actin and GAPDH are both fine.
Q: What is the theoretical molecular weight for Beta-Actin antibodies?

A: The TMW for beta Actin antibodies is approximately 42 kDa.
Q: Why is beta actin used as a loading control in blotting ?

A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
Q: Are the beta-Actin antibodies validated in Simple Western?

A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
Q: Do Beta-Actin antibodies come in lyophilized format?

A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

A: For homogenized tissue, beta-actin and GAPDH are both fine.
Q: What is the theoretical molecular weight for Beta-Actin antibodies?

A: The TMW for beta Actin antibodies is approximately 42 kDa.
Q: Why is beta actin used as a loading control in blotting ?

A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.