Recombinant Human beta-Catenin His (C-Term) Protein

Name: Recombinant Human beta-Catenin His (C-Term) Protein
Brand: Novus Biologicals
SKU: NBP3-18198
Price: 369 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-18198

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Source

E. coli

Tag

His (C-Term)

Applications

Bioactivity, SDS-PAGE

View all beta-Catenin Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a C-terminal 6xHis tag corresponding to the amino acids Met1-Leu781 of Human beta-Catenin.

Source: E. coli

(Accession #: XP_016861227.1)

Store at -20C to -80C long term. After reconstitution, the product is stable at -20C for 3 months, and 2-8C for up to 1 week.

Purity

>90%, by SDS-PAGE

Endotoxin Level

< 1.0 EU/ug of the protein by LAL method

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human CD31 at 1 ug/ml (100 ul/well) can bind Human beta-Catenin with a linear range of 0.3-4.9 ug/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human beta-Catenin His (C-Term) Protein

Bioactivity: Recombinant Human beta-Catenin His (C-Term) Protein [NBP3-18198] - Immobilized Human CD31 at 1 ug/mL (100 uL/well) can bind Human beta-Catenin with a linear range of 0.3-4.9ug/ml.

SDS-PAGE: Recombinant Human beta-Catenin His (C-Term) Protein [NBP3-18198]

SDS-Page: Recombinant Human beta-Catenin His (C-Term) Protein [NBP3-18198] - Recombinant Human beta-Catenin Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 100-110 kDa.

Formulation, Preparation, and Storage

NBP3-18198

Formulation	Lyophilized from a 0.22 um filtered solution of 50mM Tris, 150mM NaCl, pH 8.0
Concentration	Lyoph
Reconstitution	Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20C.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: beta-Catenin

The protein encoded by the beta Catenin gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs arenecessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion betweencells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contactinhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this proteinbinds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this geneare a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.

Alternate Names

bCatenin, CTNNB1

Gene Symbol

CTNNB1

Additional beta-Catenin Products

Product Documents for Recombinant Human beta-Catenin His (C-Term) Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human beta-Catenin His (C-Term) Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.