beta I Tubulin Antibody (2Z6P1)

Novus Biologicals | Catalog # NBP3-15928

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Canine, Monkey

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2Z6P1 expressed in HEK293
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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 352-451 of human beta I Tubulin (Q9H4B7). AVCDIPPRGLSMAATFIGNNTAIQEIFNRVSEHFSAMFKRKAFVHWYTSEGMDINEFGEAENNIHDLVSEYQQFQDAKAVLEEDEEVTEEAEMEPEDKGH

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for beta I Tubulin Antibody (2Z6P1)

Western Blot: beta I Tubulin Antibody (2Z6P1) [NBP3-15928]

Western Blot: beta I Tubulin Antibody (2Z6P1) [NBP3-15928]

Western Blot: beta I Tubulin Antibody (1C3J0) [NBP3-15928] - Western blot analysis of extracts of various cell lines, using beta I Tubulin Rabbit mAb (NBP3-15928) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: beta I Tubulin Antibody (2Z6P1) [NBP3-15928]

Immunocytochemistry/ Immunofluorescence: beta I Tubulin Antibody (2Z6P1) [NBP3-15928]

Immunocytochemistry/Immunofluorescence: beta I Tubulin Antibody (1C3J0) [NBP3-15928] - Immunofluorescence analysis of U-2 OS cells using beta I Tubulin Rabbit mAb (NBP3-15928) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: beta I Tubulin Antibody (2Z6P1) [NBP3-15928]

Immunocytochemistry/ Immunofluorescence: beta I Tubulin Antibody (2Z6P1) [NBP3-15928]

Immunocytochemistry/Immunofluorescence: beta I Tubulin Antibody (1C3J0) [NBP3-15928] - Immunofluorescence analysis of C6 cells using beta I Tubulin Rabbit mAb (NBP3-15928) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: beta I Tubulin Antibody (2Z6P1) [NBP3-15928]

Immunocytochemistry/ Immunofluorescence: beta I Tubulin Antibody (2Z6P1) [NBP3-15928]

Immunocytochemistry/Immunofluorescence: beta I Tubulin Antibody (1C3J0) [NBP3-15928] - Immunofluorescence analysis of NIH-3T3 cells using beta I Tubulin Rabbit mAb (NBP3-15928) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for beta I Tubulin Antibody (2Z6P1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: beta-I Tubulin

Microtubules are involved in a wide variety of cellular processes, including mitosis, morphogenesis, plateletformation, and mobility of cilia and flagella. Circulating platelets carry a single marginal microtubule coil that iswound in 8 to 12 turns and is responsible for platelet shape. TUBB1 is the major beta-tubulin expressed in plateletsand megakaryocytes and is required for optimal platelet assembly (Wang et al., 1986 (PubMed 3782288); Schulze et al.,2004 (PubMed 15315966)).(supplied by OMIM)

Long Name

Tubulin, Beta 1 Class VI

Alternate Names

betaI Tubulin, TUBB1

Gene Symbol

TUBB1

Additional beta-I Tubulin Products

Product Documents for beta I Tubulin Antibody (2Z6P1)

Certificate of Analysis

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Product Specific Notices for beta I Tubulin Antibody (2Z6P1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

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