Beta Ig-h3/TGFBI Antibody (JM24-53)
Novus Biologicals | Catalog # NBP2-67186
Recombinant Monoclonal Antibody
![Western Blot: Beta Ig-h3/TGFBI Antibody (JM24-53) [NBP2-67186]](https://resources.rndsystems.com/images/products/Beta-Ig-h3-TGFBI-Antibody-JM24-53-Western-Blot-NBP2-67186-img0005.jpg "Western Blot: Beta Ig-h3/TGFBI Antibody (JM24-53) [NBP2-67186]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JM24-53 expressed in HEK293
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Product Specifications
Immunogen
Synthetic peptide within Human Beta Ig-h3/TGFBI aa 93-142 / 683. (SwissProt: Q15582 Human; SwissProt: P82198 Mouse; SwissProt: D4A8G5 Rat)
Localization
Secreted.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Beta Ig-h3/TGFBI Antibody (JM24-53)
Western Blot: Beta Ig-h3/TGFBI Antibody (JM24-53) [NBP2-67186]
Western Blot: Beta Ig-h3/TGFBI Antibody (JM24-53) [NBP2-67186] - Analysis of TGFBI on mouse colon (1) and mouse eye (2) tissue lysate using anti-TGFBI antibody at 1/1,000 dilution.![Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody (JM24-53) [NBP2-67186]](https://resources.rndsystems.com/images/products/Beta-Ig-h3-TGFBI-Antibody-JM24-53-Immunohistochemistry-Paraffin-NBP2-67186-img0004.jpg "Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody (JM24-53) [NBP2-67186]")
Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody (JM24-53) [NBP2-67186]
Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody (JM24-53) [NBP2-67186] - Analysis of paraffin-embedded human uterus tissue using anti-TGFBI antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody (JM24-53) [NBP2-67186]](https://resources.rndsystems.com/images/products/Beta-Ig-h3-TGFBI-Antibody-JM24-53-Immunohistochemistry-Paraffin-NBP2-67186-img0001.jpg "Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody (JM24-53) [NBP2-67186]")
Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody (JM24-53) [NBP2-67186]
Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody (JM24-53) [NBP2-67186] - Analysis of paraffin-embedded human colon cancer tissue using anti-TGFBI antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody (JM24-53) [NBP2-67186]](https://resources.rndsystems.com/images/products/Beta-Ig-h3-TGFBI-Antibody-JM24-53-Immunohistochemistry-Paraffin-NBP2-67186-img0002.jpg "Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody (JM24-53) [NBP2-67186]")
Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody (JM24-53) [NBP2-67186]
Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody (JM24-53) [NBP2-67186] - Analysis of paraffin-embedded human liver cancer tissue using anti-TGFBI antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody (JM24-53) [NBP2-67186]](https://resources.rndsystems.com/images/products/Beta-Ig-h3-TGFBI-Antibody-JM24-53-Immunohistochemistry-Paraffin-NBP2-67186-img0003.jpg "Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody (JM24-53) [NBP2-67186]")
Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody (JM24-53) [NBP2-67186]
Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody (JM24-53) [NBP2-67186] - Analysis of paraffin-embedded human lung cancer tissue using anti-TGFBI antibody. Counter stained with hematoxylin.Applications for Beta Ig-h3/TGFBI Antibody (JM24-53)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: beta IG-H3
Long Name
TGF-beta Induced Gene H3
Alternate Names
beta IGH3, Kerato-epithelin, RGD-CAP, TGFBI, TGFBIP
Gene Symbol
TGFBI
Additional beta IG-H3 Products
Product Documents for Beta Ig-h3/TGFBI Antibody (JM24-53)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Beta Ig-h3/TGFBI Antibody (JM24-53)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Beta Ig-h3/TGFBI Antibody (JM24-53)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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