Beta Ig-h3/TGFBI Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-88606
![Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606]](https://resources.rndsystems.com/images/products/Beta-Ig-h3-TGFBI-Antibody-Immunohistochemistry-Paraffin-NBP1-88606-img0031.jpg "Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606]")
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Key Product Details
Validated by
Orthogonal Validation, Independent Antibodies
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Mouse (93%), Rat (92%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DHHATNGVVHLIDKVISTITNNIQQIIEIEDTFETLRAAVAASGLNTMLEGNGQYTLLAPTNEAFEKIPSETLNRILGDPEALRDLLNNHILKSAMCAEAIVAGLSVETLEGTTLEVGCSGDMLTINGKAIISNKD
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Beta Ig-h3/TGFBI Antibody - BSA Free
![Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606]](https://resources.rndsystems.com/images/products/Beta-Ig-h3-TGFBI-Antibody-Immunohistochemistry-Paraffin-NBP1-88606-img0025.jpg "Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606]")
Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606]
Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606] - Staining of human gastrointestinal, pancreas, placenta and skin using Anti-Beta Ig-h3/TGFBI antibody NBP1-88606 (A) shows similar protein distribution across tissues to independent antibody NBP2-48534 (B).![Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606]](https://resources.rndsystems.com/images/products/Beta-Ig-h3-TGFBI-Antibody-Immunohistochemistry-Paraffin-NBP1-88606-img0019.jpg "Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606]")
Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606]
Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606] - Staining of human placenta shows moderate to strong membranous positivity in stromal cells.![Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606]](https://resources.rndsystems.com/images/products/Beta-Ig-h3-TGFBI-Antibody-Immunohistochemistry-Paraffin-NBP1-88606-img0026.jpg "Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606]")
Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606]
Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606] - Staining of human eye shows moderate extracellular positivity in cornea.![Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606]](https://resources.rndsystems.com/images/products/Beta-Ig-h3-TGFBI-Antibody-Immunohistochemistry-Paraffin-NBP1-88606-img0027.jpg "Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606]")
Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606]
Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606] - Staining of human gastrointestinal shows moderate positivity in extracellular matrix of glandular cells.![Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606]](https://resources.rndsystems.com/images/products/Beta-Ig-h3-TGFBI-Antibody-Immunohistochemistry-Paraffin-NBP1-88606-img0028.jpg "Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606]")
Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606]
Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.![Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606]](https://resources.rndsystems.com/images/products/Beta-Ig-h3-TGFBI-Antibody-Immunohistochemistry-Paraffin-NBP1-88606-img0029.jpg "Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606]")
Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606]
Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606] - Staining of human skin shows moderate positivity in extracellular matrix of dermis.![Simple Western: Beta Ig-h3/TGFBI Antibody [NBP1-88606]](https://resources.rndsystems.com/images/products/Beta-Ig-h3-TGFBI-Antibody-Simple-Western-NBP1-88606-img0005.jpg "Simple Western: Beta Ig-h3/TGFBI Antibody [NBP1-88606]")
Simple Western: Beta Ig-h3/TGFBI Antibody [NBP1-88606]
Simple Western: Beta Ig-h3/TGFBI Antibody [NBP1-88606] - Simple Western lane view shows a specific band for Beta Ig-h3/TGFBI in 0.2 mg/ml of U-251 MG lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation systems.![Simple Western: Beta Ig-h3/TGFBI Antibody [NBP1-88606]](https://resources.rndsystems.com/images/products/Beta-Ig-h3-TGFBI-Antibody-Simple-Western-NBP1-88606-img0006.jpg "Simple Western: Beta Ig-h3/TGFBI Antibody [NBP1-88606]")
Simple Western: Beta Ig-h3/TGFBI Antibody [NBP1-88606]
Simple Western: Beta Ig-h3/TGFBI Antibody [NBP1-88606] - Electropherogram image of the corresponding Simple Western lane view. Beta Ig-h3/TGFBI antibody was used at 1:25 dilution on U-251 MG lysate(s) respectively.Applications for Beta Ig-h3/TGFBI Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Simple Western
1:25
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in U-251 MG lysates, separated by Size, antibody dilution of 1:25
See Simple Western Antibody Database for Simple Western validation: Tested in U-251 MG lysates, separated by Size, antibody dilution of 1:25
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: beta IG-H3
Long Name
TGF-beta Induced Gene H3
Alternate Names
beta IGH3, Kerato-epithelin, RGD-CAP, TGFBI, TGFBIP
Gene Symbol
TGFBI
Additional beta IG-H3 Products
Product Documents for Beta Ig-h3/TGFBI Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Beta Ig-h3/TGFBI Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Beta Ig-h3/TGFBI Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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