beta Tubulin Antibody (1B12) - BSA Free

Novus Biologicals | Catalog # NBP2-50061

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Single Cell Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1B12

Format

BSA Free
View all beta Tubulin Primary Antibodies »

Product Specifications

Immunogen

Pig brain beta Tubulin preparation. [UniProt# Q767L7]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta Tubulin Antibody (1B12) - BSA Free

Western Blot: beta Tubulin Antibody (1B12) [NBP2-50061]

Western Blot: beta Tubulin Antibody (1B12) [NBP2-50061]

Western Blot: beta Tubulin Antibody (1B12) [NBP2-50061] - Western blot analysis of whole tissue or cell lysates using mouse mAb to Beta-tubulin, NBP2-50061, dilution 1:10,000 in green: [1] protein standard (red), [2] adult rat brain, [3] adult mouse brain, [4] NIH-3T3 cells, [5] HEK293 cells, [6] HeLa cells, [7] SH-SY5Y cells. The strong clean band with apparent SDS-PAGE molecular weight of ~50 kDa corresponds to the Beta-tubulin proteins.
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (1B12) [NBP2-50061]

Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (1B12) [NBP2-50061]

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (1B12) [NBP2-50061] - HeLa cells stained with NBP2-50061 in green, and chicken antibody to Lamin A+C in red (NBP2-25152), and DNA in blue. NBP2-50061 reveals strong microtubular staining in the cytoplasm of HeLa cells, while the Lamin A+C localizes in the nuclear membrane.
Immunofluorescence: beta Tubulin Antibody (1B12) [NBP2-50061]

Immunofluorescence: beta Tubulin Antibody (1B12) [NBP2-50061]

Immunofluorescence: beta Tubulin Antibody (1B12) [NBP2-50061] - Immunofluorescent analysis of HeLa cells stained with NBP2-50061, dilution 1:5,000 in green, and costained with chicken pAb to the nucleic acid binding protein FOX2, dilution 1:1,000 in red. Blue is DAPI staining of nuclear DNA. NBP2-50061 produces strong staining of microtubules in the cytoplasm, while the FOX2 antibody labels the nuclei of HeLa cells.

Applications for beta Tubulin Antibody (1B12) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:5000

Western Blot

1:5000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Format

BSA Free

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: beta Tubulin

Beta Tubulin antibodies are useful as loading controls for Western Blotting and ELISA, as well as in studies invlolving cell structure and division. Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.

Long Name

Tubulin beta chain

Alternate Names

OK/SW-cl.56, TUBB, TUBB5

Gene Symbol

TUBB

UniProt

P07437 (Human)

Additional beta Tubulin Products

Product Documents for beta Tubulin Antibody (1B12) - BSA Free

Certificate of Analysis

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Product Specific Notices for beta Tubulin Antibody (1B12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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