BID Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56106

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Gerbil

Cited:

Human, Mouse, Rat, Canine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Western Blot, In vivo assay, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all BID Primary Antibodies »

Product Specifications

Immunogen

Full-length recombinant mouse Bid protein was used as immunogen.

Specificity

Full-length Bid is known to undergo cleavage/truncation (reviewed in Yin, 2006). Bid was initially found to be cleaved and activated by capspase-8 following death receptor activation. The term "Bid" was first used to described the caspase-8 cleaved/truncated C-terminal Bid. Bid can be also cleaved by other proteases such as Granzyme B, calpains and cathepsin. The C-terminal portion is considered to be the active Bid moiety. This active form can translocate from the cytosol to the mitochondria. Therefore, the appearance of Bid in the mitochondria is considered to be an indication of active Bid. However, it should also be noted that Bid has been shown to translocate to the mitochondria without cleavage in some model systems. The proteolytic cleavage of Bid usually occurs in the unstructured loop region between the alpha 2 and alpha 3 helices, which is between amino acids (aa) 41 and 79 of Bid. For example, the caspase-8/3 cleavage site is at 60 (human) and 59 (mouse). It should be noted that after cleavage, the smaller N-terminal portion of Bid is not necessarily separated from the larger C-terminal portion. The exact size of the C-terminal cleavage products depends on the Bid cleavage sites; generally 11-15 kDa C-truncated cleavage forms are generated. Shorter C-terminal Bid cleavage products may result from protease cleavage that involves both caspases and other proteases. Full length Bid is quite stable, but cleaved/truncated forms may have a short half-life (less than 1.5 h) and degrade rapidly. Therefore, a time course may be useful when detecting Bid cleavage fragments.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BID Antibody - BSA Free

Western Blot: BID Antibody [NB100-56106]

Western Blot: BID Antibody [NB100-56106]

Western Blot: BID Antibody [NB100-56106] - Analysis of Bid in normal mouse tissues using this antibody. BID = recombinant Bid. Arrowheads indicate the positions of the full-length (uncleaved) ~22 kDa Bid and the ~15 kDa truncated form of Bid (tBid) typical of the caspase-cleavage. Additional bands representing partial Bid degradation products are indicated by asterisks (*).
Immunohistochemistry-Paraffin: BID Antibody [NB100-56106]

Immunohistochemistry-Paraffin: BID Antibody [NB100-56106]

Immunohistochemistry-Paraffin: BID Antibody [NB100-56106] - Two cores from a human ovarian carcinoma formalin-fixed, paraffin-embedded tissue microarray demonstrate the variable expression of Bid protein. The sections were stained with Bid antibody at 1:2000 with hematoxylin-eosin counterstain. Section A shows very weak staining while section B stains much more strongly.
Western Blot: BID Antibody [NB100-56106]

Western Blot: BID Antibody [NB100-56106]

Western Blot: BID Antibody [NB100-56106] - Analysis of BID in NRK whole cell lysate using anti-BID antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: BID Antibody [NB100-56106]

Immunohistochemistry-Paraffin: BID Antibody [NB100-56106]

Immunohistochemistry-Paraffin: BID Antibody [NB100-56106] - Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue array stained for Bid expression using this antibody at 1:2000. Hematoxylin-eosin counterstain. Variable Bid expression is seen between patient samples.
BID Antibody - BSA Free

Western Blot: BID Antibody - BSA Free [NB100-56106] -

Pur attenuates Cd-induced apoptosis via inhibiting the Fas/FasL-mediated mitochondrial apoptotic pathway in primary cortical neurons in vitro. Primary cortical neurons were pretreated with 100 μM Pur for 1 h, followed by 10 μM Cd exposure for 12 h. (A) The levels of Fas, FasL, FADD, and Cleaved Caspase-8; (B) ratio of tBID/BID; and (C) the levels of Cleaved Caspase-9, Cleaved Caspase-3, and Cleaved PARP1 were detected by Western blot. Left panel: representative Western blot image; right panel: quantitative analysis (mean +/- SD; n = 3). The means labeled without a common letter (a, b, c) are significantly different, p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34356602), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
BID Antibody - BSA Free

Western Blot: BID Antibody - BSA Free [NB100-56106] -

Pur attenuates Cd-induced apoptosis via inhibiting the Fas/FasL-mediated mitochondrial apoptotic pathway in the rat cerebral cortex in vivo. Rats were treated with Cd (50 mg/L) and/or Pur (200 mg/kg) for 90 days. (A) The levels of Fas, FasL, FADD, and Cleaved Caspase-8; (B) ratio of tBID/BID; and (C) the levels of Cleaved Caspase-9, Cleaved Caspase-3, and Cleaved PARP1 were detected by Western blot. Left panel: representative Western blot image; right panel, quantitative analysis (mean +/- SD, n = 3). The means labeled without a common letter (a, b, c) are significantly different, p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34356602), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for BID Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Frozen

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:50-1:200

Western Blot

1:1000-1:2000

Reviewed Applications

Read 1 review rated 5 using NB100-56106 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BID

The Bcl-2 family of apoptosis-related genes plays central roles in regulating apoptotic pathways (reviewed in Thomadaki and Scorilas, 2006). Regulation of cell death through apoptosis is critical for the maintenance of homeostasis, defense against infectious agents, and normal development. Bcl-2 family proteins regulate apoptosis primarily through the regulation of mitochondrial outer membrane permeability. In mammals, the family consists of both prosurvival (antiapoptotic) and proapoptotic (prodeath) members. Cellular homeostasis is thought to be dependent on a balance between the actions of prosurvival and proapoptotic proteins. Bcl-2 family proteins can be divided into 3 main subfamilies on the basis of their function and the content of their Bcl-2 homology (BH) domains, for example: 1) Prosurvival: Bcl-2, Bcl-XL, Bcl-W, A1, and Mcl-1 2) Proapoptotic (multidomain): Bax, Bak, and Bok. 3) BH3-only (proapoptotic): Bad, Bcl-XS, Bid, Bik, Bim, Blk, Bmf, Bnip, Noxa, and Puma. Prosurvival members inhibit cells from undergoing apoptosis, whereas proapoptotic and BH3-only subfamily members promote apoptosis. There are 4 BH domains (1-4) conserved among Bcl-2 family proteins. The BH domains are important for function as well as for heterodimerization between family members. Typical prosurvival family members have all four BH domains (1-4), whereas proapoptotic (multidomain) members have BH1, 2 and 3 domains and BH3-only members have only the BH3 domain. Overall, the relative ratio of prosurvival and proapoptotic proteins determines the suseptibility of a cell to various apoptotic stimuli. Many Bcl-2 family proteins are differentially expressed in various malignancies and some are useful prognostic biomarkers. Prosurvival proteins are often elevated in diverse cancers and have the potential to confer resistance to both endogenous cell death stimuli and cancer treatments. Alterations in the ratio or levels of Bcl-2 family proteins have been also associated with nonmalignant diseases including neurodegenerative diseases, autoimmune diseases, AIDs, Down's syndrome, cardiovascular diseases, diabetes, glomerulonephritis, and muscular dystrophy. NB100-56106 recognizes Bid (approx. 19-23 kDa) and cleaved/truncated forms of Bid. The C-terminal cleaved/truncated form (11-15 kDa) of Bid is often referred to as tBid in the literature (reviewed in Yin, 2006).

Long Name

BH3 Interacting Domain Death Agonist

Alternate Names

apoptic death agonist, BH3 interacting domain death agonist, BH3-interacting domain death agonist, BID isoform ES(1b), BID isoform L(2), BID isoform Si6, desmocollin type 4, FP497, Human BID coding sequence, MGC15319, MGC42355, p22 BID, tbid

Entrez Gene IDs

637 (Human); 12122 (Mouse); 64625 (Rat)

Gene Symbol

BID

Additional BID Products

Product Documents for BID Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for BID Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for BID Antibody - BSA Free

Customer Reviews for BID Antibody - BSA Free (1)

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Customer Images

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: NRK whole cell lysate
    Species: Rat
    Verified Customer | Posted 05/22/2015
    Western Blot of BID in NRK cells
    BID Antibody - BSA Free NB100-56106

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Protocols

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FAQs

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Associated Pathways

Apoptosis Signaling Pathway
Apoptosis Signaling Pathway Thumbnail