Birch pollen Bet V1 Antibody (BV16) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-81093

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Plant

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # BV16

Format

Azide and BSA Free
View all Birch pollen Bet V1 Primary Antibodies »

Product Specifications

Immunogen

Birch pollen Bet V1-A, a major allergen of the European white birch.

Reactivity Notes

Birch (Betula pendula)

Specificity

This antibody binds to Birch pollen Bet V1.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for Birch pollen Bet V1 Antibody (BV16) - Chimeric - Azide and BSA Free

ELISA: Birch pollen Bet V1 Antibody (BV16) - Chimeric - Azide and BSA Free [NBP2-81093]

ELISA: Birch pollen Bet V1 Antibody (BV16) - Chimeric - Azide and BSA Free [NBP2-81093]

ELISA: Birch pollen Bet V1 Antibody (BV16) [NBP2-81093] - Binding curve of [NBP2-81093] to Bet v 1. ELISA Plate coated with Bet v 1-A at a concentration of 2 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using NBP2-81093. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody was used. Absorbance at 450nm does not reach a plateau but falls slightly before increasing once more. This is suggestive of two binding sites on Bet v 1-A for which NBP2-81093 has differing affinities.

Applications for Birch pollen Bet V1 Antibody (BV16) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. This antibody binds to Bet v 1 via an epitope commonly used by allergogenic IgE. Fab versions of this antibody have been used in co-crystallization studies of Bet v 1.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: Birch pollen Bet V1

Alternate Names

Allergen Bet v I-A, Allergen=Bet v 1-A, BETVI, BETVIA, Major pollen allergen Bet v 1-A

Additional Birch pollen Bet V1 Products

Product Documents for Birch pollen Bet V1 Antibody (BV16) - Chimeric - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Birch pollen Bet V1 Antibody (BV16) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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