Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3E9

Format

Azide and BSA Free
View all BMP-1/PCP Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 225-617 of human BMP1(NP_001190) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

80.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse BMP-1/PCP Antibody (OTI3E9) - Azide and BSA Free (NBP2-46579) is a monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for BMP-1/PCP Antibody (OTI3E9) - Azide and BSA Free

Western Blot: BMP-1/PCP Antibody (OTI3E9)Azide and BSA Free [NBP2-70255]

Western Blot: BMP-1/PCP Antibody (OTI3E9)Azide and BSA Free [NBP2-70255]

Western Blot: BMP-1/PCP Antibody (OTI3E9) - Azide and BSA Free [NBP2-70255] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BMP1.
Immunocytochemistry/ Immunofluorescence: BMP-1/PCP Antibody (OTI3E9) - Azide and BSA Free [NBP2-70255]

Immunocytochemistry/ Immunofluorescence: BMP-1/PCP Antibody (OTI3E9) - Azide and BSA Free [NBP2-70255]

Immunocytochemistry/Immunofluorescence: BMP-1/PCP Antibody (OTI3E9) - Azide and BSA Free [NBP2-70255] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY BMP1.
Western Blot: BMP-1/PCP Antibody (OTI3E9)Azide and BSA Free [NBP2-70255]

Western Blot: BMP-1/PCP Antibody (OTI3E9)Azide and BSA Free [NBP2-70255]

Western Blot: BMP-1/PCP Antibody (OTI3E9) - Azide and BSA Free [NBP2-70255] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)

Applications for BMP-1/PCP Antibody (OTI3E9) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:4000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-1/PCP

Bone morphogenetic protein 1 (BMP1) is a 986 amino acid member of the bone morphogenetic protein (BMP) family. It was initially discovered for its role to induce cartilage and bone formation. It is a metalloprotease that cleaves the C-terminus of procollagen I, II and III while other BMPs are part of the TGF beta superfamily. It may play a role in herniation, congestive heart failure, breast cancer, myelofibrosis with myeloid metaplasia and infection by HIV-1.

Long Name

Bone Morphogenetic Protein 1

Alternate Names

BMP1, PCP

Gene Symbol

BMP1

Additional BMP-1/PCP Products

Product Documents for BMP-1/PCP Antibody (OTI3E9) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for BMP-1/PCP Antibody (OTI3E9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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