Recombinant Human BMP-7 Protein

Novus Biologicals | Catalog # NBP2-34933

Novus Biologicals
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Key Product Details

Source

E. coli

Accession Number

P18075

Applications

Western Blot, ELISA, SDS-PAGE
View all BMP-7 Proteins and Enzymes »

Product Specifications

Description

A monomeric, non-glycosylated polypeptide chain containing 139 amino acids corresponding to BMP-7 Source: E. coli

Uniprot ID: P18075

Amino Acid Sequence: STGSKQRSQN RSKTPKNQEA LRMANVAENS SSDQRQACKK HELYVSFRDL GWQDWIIAPE GYAAYYCEGE CAFPLNSYMN ATNHAIVQTL VHFINPETVP KPCCAPTQLN AISVLYFDDS SNVILKKYRN MVVRACGCH

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of BMP-7 as determined by LAL method.

Predicted Molecular Mass

15.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

1. Molecular standard (Western, ELISA) in studying secreted BMP-7;
2. Preparing antibodies for BMP-7 monomer;
3. Molecule standard in detecting secreted BMP-7 in reduced SDS-PAGE.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human BMP-7 Protein

SDS-PAGE: Recombinant Human BMP-7 Protein [NBP2-34933]

SDS-PAGE: Recombinant Human BMP-7 Protein [NBP2-34933]

SDS-Page: Human BMP-7 Protein [NBP2-34933]

Formulation, Preparation, and Storage

NBP2-34933
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 30 % acetonitrile, 0.1 % TFA.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in 10 mM HAc to a concentration of 0.1-1.0mg/mL.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-7

Bone morphogenetic protein 7 (BMP-7), also known as osteogenic protein 1 (OP-1), is a widely expressed TGF-b superfamily member. Human BMP-7 is synthesized with a propeptide and a growth factor domain. It is secreted as a tetramer that consists of two cleaved propeptides and two disulfide-linked growth factor domains. Mature BMP-7 can also form disulfide-linked heterodimers with BMP-2 or BMP-4. Secreted BMP-7 is immobilized in the ECM through interactions with fibrillin. BMP-7 signals through complexes of type 2 receptors (Activin RIIA, Activin RIIB, and BMPR-II) and type 1 receptors (Activin RIA, BMPR-IA, and BMPR-IB). BMP-7 promotes new bone formation and nephron development, inhibits the branching of prostate epithelium, and antagonizes epithelial-mesenchymal transition (EMT). In disease, BMP-7 inhibits tumor growth and metastasis, ameliorates fibrotic damage in nephritis, and promotes neuroregeneration following brain ischemia.

Long Name

Bone Morphogenetic Protein 7

Alternate Names

BMP7, OP-1

Gene Symbol

BMP7

UniProt

P18075

Additional BMP-7 Products

Product Documents for Recombinant Human BMP-7 Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human BMP-7 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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