BNIP2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88559

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all BNIP2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VELKEEWQDEDFPIPLPEDDSIEADILAITGPEDQPGSLEVNGNKVRKKLMAPDISLTLDPSDGSVLSDDLDESGEIDLDGLDTPSENSNEFEWEDDLPKPKTTEVIRKGSITEYTAAEEKEDGRRWRMFRIGEQDHRV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (88%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BNIP2 Antibody - BSA Free

Immunohistochemistry-Paraffin: BNIP2 Antibody [NBP1-88559]

Immunohistochemistry-Paraffin: BNIP2 Antibody [NBP1-88559]

Immunohistochemistry-Paraffin: BNIP2 Antibody [NBP1-88559] - Staining of human pancreas shows very weak cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: BNIP2 Antibody [NBP1-88559]

Immunohistochemistry-Paraffin: BNIP2 Antibody [NBP1-88559]

Immunohistochemistry-Paraffin: BNIP2 Antibody [NBP1-88559] - Staining of human kidney shows moderate cytoplasmic positivity in cells in glomeruli.
Immunohistochemistry-Paraffin: BNIP2 Antibody [NBP1-88559]

Immunohistochemistry-Paraffin: BNIP2 Antibody [NBP1-88559]

Immunohistochemistry-Paraffin: BNIP2 Antibody [NBP1-88559] - Staining of human lymph node shows strong cytoplasmic positivity in non-germinal center cells.
Immunohistochemistry-Paraffin: BNIP2 Antibody [NBP1-88559]

Immunohistochemistry-Paraffin: BNIP2 Antibody [NBP1-88559]

Immunohistochemistry-Paraffin: BNIP2 Antibody [NBP1-88559] - Staining of human placenta show strong cytoplasmic positivity in trophoblastic cells.
BNIP2 Antibody - BSA Free Western Blot: BNIP2 Antibody - BSA Free [NBP1-88559]

Western Blot: BNIP2 Antibody - BSA Free [NBP1-88559]

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4
BNIP2 Antibody - BSA Free Western Blot: BNIP2 Antibody - BSA Free [NBP1-88559]

Western Blot: BNIP2 Antibody - BSA Free [NBP1-88559]

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Applications for BNIP2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BNIP2

The adenovirus E1B protein is a viral homolog of the Bcl-2 family of proteins that are involved in regulating cell death. A family of interacting proteins, which are designated Nip or Bnip and include BNIP-1, BNIP-2, BNIP-3 and Nix, associate with both the E1B protein and Bcl-2 proteins to mediate apoptotic signaling. BNIP-1 contains a hydrophobic transmembrane domain, which enables its localization to the nuclear envelope, endoplasmic recticulum and mitochondria. BNIP-2, (previously designated Nip2 and Nip21 in human and mouse respectively), shares homology with the non-catalytic domain of Cdc42 GTPase-activating protein (Cdc42GAP). Through binding to Cdc42GAP, BNIP-2 enhances the GTPase activity of Cdc42GAP, facilitating the hydrolysis of GTP bound to Cdc42 and thereby, mediating the signaling pathways involving receptor kinases, small GTPases and apoptotic proteins. Nix, which is also designated Nip3L or Bnip3L, is highly related to BNIP-3, and both proteins localize to the mitochondria where they associate with Bcl-2 proteins.

Alternate Names

BCL2/adenovirus E1B 19 kDa protein-interacting protein 2, BCL2/adenovirus E1B 19kDa interacting protein 2, BCL2/adenovirus E1B 19kD-interacting protein 2, BNIP-2, NIP2

Gene Symbol

BNIP2

Additional BNIP2 Products

Product Documents for BNIP2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for BNIP2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for BNIP2 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review BNIP2 Antibody - BSA Free and earn rewards!

Have you used BNIP2 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies