Bone marrow stromal cell antigen 1/CD157 Antibody (SY11B5) - BSA Free
Novus Biologicals | Catalog # NBP2-37711
![Flow Cytometry: Bone marrow stromal cell antigen 1/CD157 Antibody (SY11B5) - BSA Free [NBP2-37711]](https://beta-resources.rndsystems.com/images/products/Bone-marrow-stromal-cell-antigen-1-CD157-Antibody-SY11B5-Flow-Cytometry-NBP2-37711-img0004.jpg "Flow Cytometry: Bone marrow stromal cell antigen 1/CD157 Antibody (SY11B5) - BSA Free [NBP2-37711]")
Key Product Details
Species Reactivity
Human, Primate
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunoprecipitation, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SY11B5
Format
BSA Free
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Product Specifications
Immunogen
Human CD157.
Specificity
The mouse monoclonal antibody SY11B5 recognizes CD157, an approximately 45 kDa GPI-anchored protein expressed mainly on monocytes, macrophages, granulocytes and bone marrow stromal cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Mouse Bone marrow stromal cell antigen 1/CD157 Antibody (SY11B5) - BSA Free (NBP2-37711) is a monoclonal antibody validated for use in IHC, WB, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Bone marrow stromal cell antigen 1/CD157 Antibody (SY11B5) - BSA Free
Flow Cytometry: Bone marrow stromal cell antigen 1/CD157 Antibody (SY11B5) - BSA Free [NBP2-37711]
Flow Cytometry: Bone marrow stromal cell antigen 1/CD157 Antibody (SY11B5) [NBP2-37711] - Surface staining of human peripheral blood leukocytes with anti-human CD157 (SY11B5) FITC.![Flow Cytometry: Bone marrow stromal cell antigen 1/CD157 Antibody (SY11B5) - BSA Free [NBP2-37711]](https://beta-resources.rndsystems.com/images/products/Bone-marrow-stromal-cell-antigen-1-CD157-Antibody-SY11B5-Flow-Cytometry-NBP2-37711-img0001.jpg "Flow Cytometry: Bone marrow stromal cell antigen 1/CD157 Antibody (SY11B5) - BSA Free [NBP2-37711]")
Flow Cytometry: Bone marrow stromal cell antigen 1/CD157 Antibody (SY11B5) - BSA Free [NBP2-37711]
Flow Cytometry: Bone marrow stromal cell antigen 1/CD157 Antibody (SY11B5) [NBP2-37711] - Surface staining of human peripheral blood leukocytes with anti-human CD157 (SY11B5) APC.![Flow Cytometry: Bone marrow stromal cell antigen 1/CD157 Antibody (SY11B5) - BSA Free [NBP2-37711]](https://beta-resources.rndsystems.com/images/products/Bone-marrow-stromal-cell-antigen-1-CD157-Antibody-SY11B5-Flow-Cytometry-NBP2-37711-img0002.jpg "Flow Cytometry: Bone marrow stromal cell antigen 1/CD157 Antibody (SY11B5) - BSA Free [NBP2-37711]")
Flow Cytometry: Bone marrow stromal cell antigen 1/CD157 Antibody (SY11B5) - BSA Free [NBP2-37711]
Flow Cytometry: Bone marrow stromal cell antigen 1/CD157 Antibody (SY11B5) [NBP2-37711] - Surface staining of human peripheral blood leukocytes with anti-human CD157 (SY11B5) purified / GAM-APC.![Flow Cytometry: Bone marrow stromal cell antigen 1/CD157 Antibody (SY11B5) - BSA Free [NBP2-37711]](https://beta-resources.rndsystems.com/images/products/Bone-marrow-stromal-cell-antigen-1-CD157-Antibody-SY11B5-Flow-Cytometry-NBP2-37711-img0003.jpg "Flow Cytometry: Bone marrow stromal cell antigen 1/CD157 Antibody (SY11B5) - BSA Free [NBP2-37711]")
Flow Cytometry: Bone marrow stromal cell antigen 1/CD157 Antibody (SY11B5) - BSA Free [NBP2-37711]
Flow Cytometry: Bone marrow stromal cell antigen 1/CD157 Antibody (SY11B5) [NBP2-37711] - Surface staining of human peripheral blood leukocytes with anti-human CD157 (SY11B5) PE.Applications for Bone marrow stromal cell antigen 1/CD157 Antibody (SY11B5) - BSA Free
Application
Recommended Usage
Flow Cytometry
1-12 ug/ml
Immunohistochemistry
5-10 ug/ml
Immunohistochemistry-Frozen
1:10-1:500
Immunoprecipitation
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
Western Blotting - non-reducing conditions. This antibody is CyTOF ready.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
Phosphate buffered saline (PBS), pH 7.4
Format
BSA Free
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CD157
Long Name
Cluster of Differentiation 157
Alternate Names
BP-3, BST1, cADPr hydrolase 2, CD157
Gene Symbol
BST1
Additional CD157 Products
Product Documents for Bone marrow stromal cell antigen 1/CD157 Antibody (SY11B5) - BSA Free
Product Specific Notices for Bone marrow stromal cell antigen 1/CD157 Antibody (SY11B5) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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