Brachyury Antibody (6C12) - BSA Free

Novus Biologicals | Catalog # NBP2-37473

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6C12

Format

BSA Free
View all Brachyury Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Brachyury (AA: 218-352) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

47.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Brachyury Antibody (6C12) - BSA Free

Western Blot: Brachyury Antibody (6C12)BSA Free [NBP2-37473]

Western Blot: Brachyury Antibody (6C12)BSA Free [NBP2-37473]

Western Blot: Brachyury Antibody (6C12) [NBP2-37473] - Analysis using T mAb against HEK293 (1) and T (AA: 218-352)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: Brachyury Antibody (6C12) - BSA Free [NBP2-37473]

Flow Cytometry: Brachyury Antibody (6C12) - BSA Free [NBP2-37473]

Flow Cytometry: Brachyury Antibody (6C12) [NBP2-37473] - Analysis of A549 cells using T mouse mAb (green) and negative control (red).
Western Blot: Brachyury Antibody (6C12)BSA Free [NBP2-37473]

Western Blot: Brachyury Antibody (6C12)BSA Free [NBP2-37473]

Western Blot: Brachyury Antibody (6C12) [NBP2-37473] - Analysis using T mAb against human T recombinant protein. (Expected MW is 40.3 kDa)
ELISA: Brachyury Antibody (6C12) - BSA Free [NBP2-37473]

ELISA: Brachyury Antibody (6C12) - BSA Free [NBP2-37473]

ELISA: Brachyury Antibody (6C12) [NBP2-37473] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for Brachyury Antibody (6C12) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Brachyury

Brachyury / Bry is encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.

Alternate Names

brachyury protein, MGC104817, Protein T, SAVA, T brachyury (mouse) homolog, T brachyury homolog, T Brachyury Transcription Factor, T, brachyury homolog (mouse), T-Box Transcription Factor T, TBXT, TF, TFT

Gene Symbol

TBXT

Additional Brachyury Products

Product Documents for Brachyury Antibody (6C12) - BSA Free

Certificate of Analysis

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Product Specific Notices for Brachyury Antibody (6C12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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