BRCA1 Antibody (BRCA1/1472) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-54337
Key Product Details
Species Reactivity
Human
Applications
ELISA, Protein Array, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # BRCA1/1472
Format
Azide and BSA Free
Loading...
Product Specifications
Immunogen
Recombinant fragment (around aa 445-620) of human BRCA1 protein (exact sequence is proprietary) (Uniprot: P38398)
Localization
Cytoplasmic and Nuclear
Marker
Breast Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53177).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for BRCA1 Antibody (BRCA1/1472) - Azide and BSA Free
Protein Array: BRCA1 Antibody (BRCA1/1472) - Azide and BSA Free [NBP2-54337]
Protein Array: BRCA1 Antibody (BRCA1/1472) - Azide and BSA Free [NBP2-54337] - Protein Array containing more than 19,000 full-length human proteins using BRCA-1 antibody (BRCA1/1472). The Z-score represents the strength of a signal that a monoclonal antibody, mAb, (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein. Z-scores are in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.Applications for BRCA1 Antibody (BRCA1/1472) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:100 - 1:2000
Application Notes
ELISA: For coating use Ab at 2-4ug/ml, order Ab without BSA.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: BRCA1
BRCA1 antibodies are useful for cancer studies.
Long Name
Breast Cancer 1
Alternate Names
BRCAI, breast and ovarian cancer susceptibility protein 1, breast and ovarian cancer sususceptibility protein, breast cancer 1, early onset, breast cancer type 1 susceptibility protein, EC 6.3.2, EC 6.3.2.-, IRIS, PNCA4, PSCP, subunit 1
Gene Symbol
BRCA1
Additional BRCA1 Products
Product Documents for BRCA1 Antibody (BRCA1/1472) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for BRCA1 Antibody (BRCA1/1472) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for BRCA1 Antibody (BRCA1/1472) - Azide and BSA Free
There are currently no reviews for this product. Be the first to review BRCA1 Antibody (BRCA1/1472) - Azide and BSA Free and earn rewards!
Have you used BRCA1 Antibody (BRCA1/1472) - Azide and BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...