BRCAA1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-26618

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot, Immunoprecipitation, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 125 and 175 of human AT rich interactive domain 4B using the numbering given in entry NP_057458.4 (GeneID 51742).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BRCAA1 Antibody - BSA Free

Western Blot: BRCAA1 Antibody [NBP1-26618]

Western Blot: BRCAA1 Antibody [NBP1-26618]

Western Blot: BRCAA1 Antibody [NBP1-26618] - Whole cell lysate from HeLa cells. ARID4B was also immunoprecipitated by rabbit anti-ARID4B antibody NBP1-26619.
Immunohistochemistry: BRCAA1 Antibody [NBP1-26618]

Immunohistochemistry: BRCAA1 Antibody [NBP1-26618]

Immunohistochemistry: BRCAA1 Antibody [NBP1-26618] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ARID4B used at a dilution of 1:1,000 ( 1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.

Applications for BRCAA1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

5-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Use in immunprecipitation and chromatin immunoprecipitation reported in scientific literature (PMID 25351346)

Reviewed Applications

Read 1 review rated 5 using NBP1-26618 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BRCAA1

ARID4B (AT-rich interactive domain-containing protein 4B) is also known as RbBP1L1 (retinoblastoma binding protein 1-like 1) and is a retinoblastoma binding protein and member of the AT-rich interaction domain (ARID) family of proteins. In addition to the ARID DNA binding domain, ARID4B also contains a chromo domain and a TUDOR domain, indicating a function in chromatin organization and protein-protein interactions with methylated protein substrates. As a subunit of the SIN3A transcriptional co-repressor, ARID4B is implicated to be involved in chromatin remodeling and epigenetic modifications. ARID4B may play a role in the pathogenesis of breast cancer and other malignancies.

Alternate Names

180 kDa Sin3-associated polypeptide, ARID domain-containing protein 4B, AT rich interactive domain 4B (RBP1- like), AT rich interactive domain 4B (RBP1-like), AT-rich interactive domain-containing protein 4B, BRCAA1DKFZp313M2420, breast cancer-associated antigen 1, Breast cancer-associated antigen BRCAA1, breast carcinoma-associated antigen, Histone deacetylase complex subunit SAP180, Rb-binding protein homolog, RBBP1L1RBP1L1BCAA, RBP1-like protein, retinoblastoma binding protein 1-like 1, Retinoblastoma-binding protein 1-like 1, SAP180MGC163290, SIN3A-associated protein 180, Sin3-associated polypeptide p180

Entrez Gene IDs

51742 (Human)

Gene Symbol

ARID4B

UniProt

Q4LE39 (Human)

Additional BRCAA1 Products

Product Documents for BRCAA1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for BRCAA1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for BRCAA1 Antibody - BSA Free

Customer Reviews for BRCAA1 Antibody - BSA Free (1)

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  • Name: rajesha rupaimoole
    Application: Immunoprecipitation
    Sample Tested: Human cancer cell line A2780, MCF7
    Species: Human
    Verified Customer | Posted 10/06/2014

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