BRD4 [p Thr204] Antibody (3V5R1)

Novus Biologicals | Catalog # NBP3-16958

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3V5R1 expressed in HEK293
View all BRD4 Primary Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around T204 of human BRD4 (NP_490597.1). ASTPP

Modification

p Thr 204

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BRD4 [p Thr204] Antibody (3V5R1)

Western Blot: BRD4 [p Thr204] Antibody (3V5R1) [NBP3-16958] - Western blot analysis of extracts of various cell lines, using (NBP3-16958) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight.C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Western Blot: BRD4 [p Thr204] Antibody (3V5R1) [NBP3-16958] - Western blot analysis of extracts of NIH/3T3 cells, using (NBP3-16958) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) for 30 minutes with NaCl (400mM) after serum-starvation 16-20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Applications for BRD4 [p Thr204] Antibody (3V5R1)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BRD4

BRD4 is encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq]

Long Name

Bromodomain Containing 4

Alternate Names

HUNK1, HUNKI, MCAP

Gene Symbol

BRD4

Additional BRD4 Products

Product Documents for BRD4 [p Thr204] Antibody (3V5R1)

Certificate of Analysis

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Product Specific Notices for BRD4 [p Thr204] Antibody (3V5R1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

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