Brevican Antibody (S294A-6) - BSA Free

Novus Biologicals | Catalog # NBP2-22401

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # S294A-6

Format

BSA Free
View all Brevican Primary Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 219-655 of rat Brevican

Localization

Extracellular Space, Extracellular Matrix, Membrane, Lipid-Anchor, GPI-Anchor

Specificity

Detects approx 140kDa (and smaller due to proteolytic cleavage).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Brevican Antibody (S294A-6) - BSA Free

Western Blot: Brevican Antibody (S294A-6) [NBP2-22401]

Western Blot: Brevican Antibody (S294A-6) [NBP2-22401]

Western Blot: Brevican Antibody (S294A-6) [NBP2-22401] - Western Blot analysis of Rat Brain Membrane showing detection of ~140 kDa Brevican protein using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6 (NBP2-22401). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug).. Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody (NBP2-22401) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~140 kDa.
Immunocytochemistry/ Immunofluorescence: Brevican Antibody (S294A-6) [NBP2-22401]

Immunocytochemistry/ Immunofluorescence: Brevican Antibody (S294A-6) [NBP2-22401]

Immunocytochemistry/Immunofluorescence: Brevican Antibody (S294A-6) [NBP2-22401] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6 (NBP2-22401). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody (NBP2-22401) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Brevican Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: Brevican Antibody (S294A-6) [NBP2-22401]

Immunocytochemistry/ Immunofluorescence: Brevican Antibody (S294A-6) [NBP2-22401]

Immunocytochemistry/Immunofluorescence: Brevican Antibody (S294A-6) [NBP2-22401] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X.
Immunocytochemistry/ Immunofluorescence: Brevican Antibody (S294A-6) [NBP2-22401]

Immunocytochemistry/ Immunofluorescence: Brevican Antibody (S294A-6) [NBP2-22401]

Immunocytochemistry/Immunofluorescence: Brevican Antibody (S294A-6) [NBP2-22401] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6 (NBP2-22401). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody (NBP2-22401) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Brevican Antibody. (D) Composite.

Applications for Brevican Antibody (S294A-6) - BSA Free

Application
Recommended Usage

Western Blot

1:1000
Application Notes
1 ug/ml of Brevican Antibody was sufficient for detection of Brevican in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Brevican

BCAN - brevican

Long Name

Brain Specific Proteoglycan in the Aggrecan Family

Alternate Names

ALPBRE, BCAN, BEHAB, CSPG7

Gene Symbol

BCAN

UniProt

P55068 (Rat)

Additional Brevican Products

Product Documents for Brevican Antibody (S294A-6) - BSA Free

Certificate of Analysis

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Product Specific Notices for Brevican Antibody (S294A-6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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