BRM Antibody

Western Blot: BRM Antibody [NB100-55308]

Western Blot: BRM Antibody [NB100-55308] - Detection of human SMARCA2/BRM by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMARCA2/BRM antibody NB100-55308 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SMARCA2/BRM was also immunoprecipitated by rabbit anti-SMARCA2/BRM antibodies NB100-55307 and NB100-55309, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).

Western Blot: BRM Antibody [NB100-55308] -

KMT9 controls expression of genes involved in the organization of organelles, cells death and cell proliferation. a Venn diagram showing overlap and number of genes/proteins in A549 cells that are differentially expressed upon RNAi mediated knock-down of KMT9 alpha (log2(fold-change) > +/- 0.26). In total, 460 targets are concomitantly up- or down-regulated on RNA and protein level upon knock-down of KMT9 alpha. Enriched GO_cellular components b and GO_biological processes c gene sets obtained for the indicated 460 KMT9 alpha -regulated target genes. d Heat map displaying mRNA levels of the 460 KMT9 alpha -regulated genes involved in cell proliferation (GO:0042127) in A549 cells treated with siControl or siKMT9 alpha #1. e RNA sequencing reads (left panel) and mass spectrometry volcano plot (right panel) for the indicated genes and proteins are represented exemplarily. f Western blot displaying expression of the target proteins indicated in e upon knock-down of KMT9 alpha in A549 cells. The indicated antibodies were used. g Quantitative real-time PCR analysis of the mRNA expression of the target genes displayed in e after knock-down of KMT9 alpha. Data represent means + standard deviation. Significance was accessed by two-tailed t test, n = 3 (TIMP2 n = 6) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32095117), licensed under a CC-BY license. Not internally tested by Novus Biologicals.