BRM Antibody

Novus Biologicals | Catalog # NB100-55309

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation, QcWestern

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all BRM Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1325 and 1375 of human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 using the numbering given in entry CAA51407.1 (GeneID 6595)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BRM Antibody

Western Blot: BRM Antibody [NB100-55309]

Western Blot: BRM Antibody [NB100-55309]

Western Blot: BRM Antibody [NB100-55309] - Detection of human SMARCA2/BRM by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMARCA2/BRM antibody NB100-55309 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SMARCA2/BRM was also immunoprecipitated by rabbit anti-SMARCA2/BRM antibodies NB100-55307 and NB100-55308, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
Immunoprecipitation: BRM Antibody [NB100-55309]

Immunoprecipitation: BRM Antibody [NB100-55309]

Immunoprecipitation: BRM Antibody [NB100-55309] - Detection of human SMARCA2/BRM by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMARCA2/BRM antibody NB100-55309 used for WB at 0.04 ug/ml. SMARCA2/BRM was also immunoprecipitated by rabbit anti-SMARCA2/BRM antibodies NB100-55307 and NB100-55308, which recognize upstream epitopes. For blotting immunoprecipitated SMARCA2/BRM, NB100-55309 was used at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 3 seconds.
QcWestern: BRM Antibody [NB100-55309]

QcWestern: BRM Antibody [NB100-55309]

QcWestern: BRM Antibody [NB100-55309] - Detection of human SMARCA2/BRM by western blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMARCA2/BRM antibody NB100-55309 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.

Applications for BRM Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BRM

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Two transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism.

Long Name

Probable global transcription activator SNF2L2

Alternate Names

BAF190B, EC 3.6.4.-, hBRM, SMARCA2, SNF2-alpha, SNF2A, SNF2L2

Entrez Gene IDs

6595 (Human)

Gene Symbol

SMARCA2

UniProt

CAA51407.1 (Human)

Additional BRM Products

Product Documents for BRM Antibody

Certificate of Analysis

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Product Specific Notices for BRM Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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