Bromodeoxyuridine/BrdU APC-conjugated Antibody

  (1 citations)     
Datasheet / CoA / SDS
Product Details
Citations (1)
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  • Specificity
    Detects Bromodeoxyuridine/BrdU.
  • Source
    Monoclonal Mouse IgG2A Clone # BU-1
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    5-iodourindine (5-IO) coupled to ovalbumin
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Intracellular Staining by Flow Cytometry
Detection of Bromodeoxyuridine/BrdU in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were treated overnight with 50 ng/mL PMA, 500 ng/mL Ionomycin, and 30 µg/mL BrdU, then stained with Mouse Anti-Bromodeoxyuridine/BrdU APC‑conjugated Monoclonal Antibody (Catalog # IC7225A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with cold, 70% ethanol for 5 minutes, DNA was denatured with 1.5M HCl for 30 minutes, and then cells were permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Bromodeoxyuridine/BrdU
Bromodeoxyuridine (BrdU) is a nucleoside analog that is incorporated into DNA in place of thymidine. BrdU differs from thymidine by virtue of the fact that BrdU shows a bromine atom substitution for a methyl group in thymidine. The detection of newly synthesized DNA containing BrdU is a commonly used measure of cell proliferation and progression through S phase of the cell cycle.
  • Alternate Names:
    5-bromo-2-deoxyuridine; BrdU; Bromodeoxyuridine; BUdr
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Human NK Cells Downregulate Zap70 and Syk in Response to Prolonged Activation or DNA Damage
    Authors: JL Pugh, N Nemat-Gorg, PJ Norman, LA Guethlein, P Parham
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A APC-conjugated Antibody

Ctrl IC003A 3  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 4
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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