Bromodeoxyuridine/BrdU Antibody

(1 citations)   
  • Specificity
    Detects Bromodeoxyuridine/BrdU.
  • Source
    Monoclonal Mouse IgG2A Clone # BU-1
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    5-iodourindine (5-IO) coupled to ovalbumin
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    0.25 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Bromodeoxyuridine/BrdU in MCF‑7 Human Cell Line. Bromodeoxyuridine/BrdU was detected in immersion fixed MCF‑7 human breast cancer cell line stimulated with BrdU using Mouse Anti-Bromodeoxyuridine/
BrdU Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB7225) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Intracellular Staining by Flow Cytometry
Detection of Bromodeoxyuridine/BrdU in Human PBMCs by Flow Cytometry Human peripheral blood mononuclear cells (PBMCs) were treated overnight with 50 ng/mL PMA, 500 ng/mL Ionomycin, and 30 µg/mL BrdU, then stained with Mouse Anti-Bromodeoxyuridine/BrdU Monoclonal Antibody (Catalog # MAB7225, filled histogram) or isotype control antibody (Catalog # MAB003, open histo­gram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with cold, 70% ethanol for 5 minutes, DNA was denatured with 1.5M HCl for 30 minutes, and then cells were permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Bromodeoxyuridine/BrdU
Bromodeoxyuridine (BrdU) is a nucleoside analog that is incorporated into DNA in place of thymidine. The detection of newly synthesized DNA containing BrdU is a commonly used measure of cell proliferation and progression through S phase of the cell cycle.
  • Alternate Names:
    5-bromo-2-deoxyuridine; BrdU; Bromodeoxyuridine; BUdr
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. PDGFRbeta expression and function in fibroblasts derived from pluripotent cells is linked to DNA demethylation.
    J. Cell. Sci., 2012;125(0):2276-87.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

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