BRPF1 Antibody (HL2064) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25595

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2064 expressed in HEK293

Format

Azide and BSA Free
View all BRPF1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human BRPF1

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BRPF1 Antibody (HL2064) - Azide and BSA Free

BRPF1 Antibody (HL2064) - Azide and BSA Free

Western Blot: BRPF1 Antibody (HL2064) - Azide and BSA Free [NBP3-25595] -

Western Blot: BRPF1 Antibody (HL2064) - Azide and BSA Free [NBP3-25595] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Peregrin antibody [HL2064] (NBP3-25595) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
BRPF1 Antibody (HL2064) - Azide and BSA Free

Western Blot: BRPF1 Antibody (HL2064) - Azide and BSA Free [NBP3-25595] -

Western Blot: BRPF1 Antibody (HL2064) - Azide and BSA Free [NBP3-25595] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Peregrin antibody [HL2064] (NBP3-25595) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
BRPF1 Antibody (HL2064) - Azide and BSA Free

Western Blot: BRPF1 Antibody (HL2064) - Azide and BSA Free [NBP3-25595] -

Western Blot: BRPF1 Antibody (HL2064) - Azide and BSA Free [NBP3-25595] - Wild-type (WT) and BRPF1 knockout (KO) 293T cell extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Peregrin antibody [HL2064] (NBP3-25595) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
BRPF1 Antibody (HL2064) - Azide and BSA Free

Immunohistochemistry-Paraffin: BRPF1 Antibody (HL2064) - Azide and BSA Free [NBP3-25595] -

Immunohistochemistry-Paraffin: BRPF1 Antibody (HL2064) - Azide and BSA Free [NBP3-25595] - Peregrin antibody [HL2064] detects Peregrin protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Peregrin stained by Peregrin antibody [HL2064] (NBP3-25595) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
BRPF1 Antibody (HL2064) - Azide and BSA Free

Western Blot: BRPF1 Antibody (HL2064) [NBP3-25595] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRPF1 antibody [HL2064] (NBP3-25595) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for BRPF1 Antibody (HL2064) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BRPF1

BRPF1 is encoded by this gene is expressed ubiquitously and at the highest level in testes and spermatogonia. The protein is localized within nuclei, and it is very similar in structure to two zinc finger proteins, AF10 and AF17. It is suggested that these proteins form a family of regulatory proteins. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

Peregrin

Alternate Names

BR140, Protein Br140

Gene Symbol

BRPF1

Additional BRPF1 Products

Product Documents for BRPF1 Antibody (HL2064) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for BRPF1 Antibody (HL2064) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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